Calcium-binding protein protocols. / Volume I. Reviews and case studies

Dedication .........................................................................................................v Preface ........................................................................................................... vii Contents of Companion Volume ..................................................................... xi Contributors..................................................................................................xiii PART I. INTRODUCTION AND REVIEWS 1 Calcium-Binding Proteins Hans J. Vogel, Richard D. Brokx, and Hui Ouyang ........................... 3 2 Calcium Robert J. P. Williams ........................................................................... 21 3 Crystal Structure of Calpain and Insights into Ca2+-Dependent Activation Zongchao Jia, Christopher M. Hosfield, Peter L. Davies, and John S. Elce .............................................................................. 51 4 The Multifunctional S100 Protein Family Claus W. Heizmann .............................................................................. 69 5 Ca2+ Binding to Proteins Containing γ-Carboxyglutamic Acid Residues Egon Persson ....................................................................................... 81 6 The Caseins of Milk as Calcium-Binding Proteins Harold M. Farrell, Jr., Thomas F. Kumosinski, Edyth L. Malin, and Eleanor M. Brown ..................................................................... 97 PART II. CALCIUM-BINDING PROTEINS: CASE STUDIES 7 Preparation of Recombinant Plant Calmodulin Isoforms Raymond E. Zielinski ........................................................................ 143 8 Isolation of Recombinant Cardiac Troponin C John A. Putkey and Wen Liu ............................................................ 151 9 Skeletal Muscle Troponin C: Expression and Purification of the Recombinant Intact Protein and Its Isolated N- and C-Domain Fragments Joyce R. Pearlstone and Lawrence B. Smillie ............................... 161 10 Purification of Recombinant Calbindin D9k Eva Thulin ........................................................................................... 175 ix 11 S100 Proteins: From Purification to Functions Jean Christophe Deloulme, Gaëlh Ouengue Mbele, and Jacques Baudier .................................................................... 185 12 Cadherins Jean-René Alattia, Kit I. Tong, Masatoshi Takeichi, and Mitsuhiko Ikura ....................................................................... 199 13 α-Lactalbumin and (Calcium-Binding) Lysozyme Katsutoshi Nitta ............................................................................. 211 14 Recombinant Annexin II Tetramer Hyoung-Min Kang, Nolan R. Filipenko, Geetha Kassam, and David M. Waisman ................................................................. 225 15 Purification and Characterization of ALG-2: A Novel Apoptosis-Linked Ca2+-Binding Protein Mingjie Zhang and Kevin W.-H. Lo .................................................. 235 16 Crystallization and Structural Details of Ca2+-Induced Conformational Changes in the EF-Hand Domain VI of Calpain Miroslaw Cygler, Pawel Grochulski, and Helen Blanchard .......... 243 17 Neurocalcin: Role in Neuronal Signaling Senadhi Vijay-Kumar and Vinod D. Kumar .................................... 261 18 Crystallization and Structure–Function of Calsequestrin ChulHee Kang, William R. Trumble, and A. Keith Dunker ............ 281 19 Use of Fluorescence Resonance Energy Transfer to Monitor Ca2+-Triggered Membrane Docking of C2 Domains Eric A. Nalefski and Joseph J. Falke .............................................. 295 20 Ca2+-Binding Mode of the C2A-Domain of Synaptotagmin Josep Rizo, Josep Ubach, and Jesús García ................................ 305 21 Study of Calcineurin Structure by Limited Proteolysis Seun-Ah Yang and Claude Klee ....................................................... 317 Index ............................................................................................................ 335