Mitochondria, 2nd Edition

Isolation and Subfractionation of Mitochondria 1. Isolation and Subfractionation of Mitochondria in Animal Cells 2. Purification and Subfractionation of Mitochondria from the Yeast Saccharomyces cerevisiae 3. Isolation and Subfractionation of Mitochondria from Plants Biochemical Assays of Mitochondrial Activity 4. Biochemical Assays of Respiratory Chain Complex Activity 5. Polarographic Assays of Respiratory Chain Complex Activity 6. Optical Imaging Techniques (Histochemical, Immunohistochemical and In Situ Hybridization Staining Methods) to Visualize Mitochondria 7. Assay of Mitochondrial ATP Synthesis in Animal Cells and Tissues 8. Measurement of the Ratio of Lactate to Pyruvate in Skin Fibroblast Cultures 9. Assays of Fatty Acid O-Oxidation Activity 10. Biochemical Assays for Mitochondrial Activity: Assays of TCA Cycle Enzymes and PDHc 11. Assays of Cardiolipin Levels 12. Measurement of VDAC Permeability in Intact Mitochondria and in Reconstituted Systems 13. Methods for Studying Fe/S Cluster Protein Maturation in Yeast Mitochondria Assays for Mitochondrial Respiratory Activity and Permeability in Living Cells 14. Imaging of Mitochondrial Polarization and Depolarization with Cationic Fluorophores 15. Biosensors for the Detection of Calcium and pH 16. Measurement of Membrane Permeability and the Permeability Transition of Mitochondria 17. Luciferase Expression for ATP Imaging: Application to Cardiac Myocytes Oxidative Stress Measurements 18. Measurement of Reactive Oxygen Species in Cells and Mitochondria 19. Measurements of the Antioxidant Enzyme Activities of Superoxide Dismutase, Catalase, and Glutathione Peroxidase 20. Methods for Measuring the Regulation of Respiration by Nitric Oxide 21. Methods for Determining the Modification of Protein Thiols by Reactive Lipids Mitochondrial Genes and Gene Expression 22. Detection of Mutations in mtDNA 23. Diagnostic Assays for Defects in mtDNA Replication and Transcription in Yeast and Humans 24. Microdissection and Analytical PCR for the Investigation of mtDNA Lesions 25. Transmitochondrial Technology in Animal Cells 26. Genetic Transformation of Saccharomyces cerevisiae and Chlamydomonas Reinhardtii Mitochondria 27. Generation of Transmitochondrial Mice: Development of Xenomitochondrial Mice to Model Neurodegenerative Diseases 28. In vivo and In organello Analysis of Mitochondrial Translation Assays for Mitochondrial Morphology and Motility 29. Visualization of Mitochondria in Budding Yeast 30. Visualization and Quantification of Mitochondrial Dynamics in Living Animal Cells 31. Cell-Free Assays for Mitochodnrial-Cytoskeletal Interactions 32. Assays to Measure Mitochondrial Fusion In Vitro and the GTPase Activity of Mitochondrial Fission Proteins Methods to Determine Protein Localization to Mitochondria 33. Electrophoretic Methods to Isolate Protein Complexes from Mitochondria 34. Analysis of Protein-Protein Interactions in Mitochondria 35. Analysis and Prediction of Mitochondrial Targeting Signals 36. Import of Proteins into Mitochondria Appendices Appendix 1: Basic Properties of Mitochondria Appendix 2: Direct and Indirect Inhibitors of Mitochondrial ATP Synthesis Appendix 3: Linearized Maps of Mitochondrial Genomes from Representative Organisms Appendix 4: Mitochondrial Genetic Codes in Various Organisms Appendix 5: Gene Products Present in Mitochondria of Yeast and Animal Cells Appendix 6: Changes in the Mitochondrial Transcriptome and Proteome Under Various Stresses and Growth Conditions