Brucella : molecular and cellular biology

List of Contributors viii Foreword xvi Chapter1 DNA Polymorphism and Taxonomy of Brucella Species Axel Cloeckaert and Nieves Vizcaino 1 Chapter2 Molecular Diagnostics of Animal Brucellosis: A Review of PCRBased Assays and Approaches Betsy J.Bricker 24 Chapter3 Polymerase Chain Reaction: A Powerful New Approach for the Diagnosis of Human Brucellosis Juan D.Colmenero, María I.Queipo-Ortuño and Pilar Morata 51 Chapter4 A Brucella melitensis Genomic Database Xavier De Bolle, Christophe Lambert, Eric Depiereux and Jean- Jacques Letesson 67 Chapter5 Comparative Genomics of Brucella melitensis, B. suis, and B. abortus Shirley M.Halling, Cynthia Gibas and Stephen Boyle 84 Chapter6 Comparative Proteomics of Brucella Species Vito G.DelVecchio and Cesar V.Mujer 102 Chapter7 Brucella Virulence: A Matter of Control Jean-Jaques Letesson and Xavier De Bolle 113 Chapter8 Brucella Lipopolysaccharide: Structure, Biosynthesis and Genetics Maite Iriarte, David González, Rose M.Delrue, Daniel Monreal, Raquel Conde, Ignacio López-Goñi, Jean-Jacques Letesson, and Ignacio Moriyón 152 Chapter9 Brucella Cyclic β-1,2-Glucans: Structure, Biosynthesis, Biological Activities and Role in Virulence Nora Iñón de Iannino and Rodolfo A.Ugalde 184 Chapter10 The Brucella BvrS/BvrR and Related Two-Component Regulatory Systems of the α-2 Proteobacteria: Common Regulatory Strategies of Animal and Plant Pathogens and Endosymbionts Ignacio López-Goñi, Lorea Manterola and Shen Q.Pan 204 Chapter11 Erythritol Metabolism and Virulence in Brucella Juan M.García-Lobo and Felix J.Sangari García 223 Chapter12 Iron Metabolism in Brucella R.Martin Roop II, Bryan H.Bellaire, Eric Anderson and James T.Paulley 236 Chapter13 Brucella Interaction with Membrane Lipids of the Host Cell Masahisa Watarai 257 Chapter14 Invasion, Intracellular Trafficking and Replication of Brucella Organisms in Professional and Non-Professional Phagocytes Edgardo Moreno and Jean-Pierre Gorvel 280 Chapter15 The Intramacrophagic Environment of Brucella spp. and Their Replicative Niche Véronique Jubier-Maurin, Séverine Loisel, Jean-Pierre Liautard, and Stephan Köhler 307 Chapter16 Host Cellular Immune Responses Against Brucella spp. Evaluated Using the Mouse Model Cynthia L.Baldwin and Radhika Goenka 335 Chapter17 Development of New Brucella Vaccines by Molecular Methods David L.Hoover, Mikeljon P.Nikolich, Mina J.Izadjoo, Richard H.Borschel, and Apurba K.Bhattacharjee 362 Chapter18 Alternative Ovine Brucellosis Vaccine: Experiences with Drug Delivery Systems Carlos Gamazo, Maite Estevan, Maribel Murillo, M.Jesús Grilló, Clara M.Marín, Montserrat Barberán, M.Mar Goñi, José M.Blasco, and Juan M.Irache 393 Index 418