Plant tissue culture : theory and practice

Preface ................................................................................................................... v Chapter 1. Introductory history ........................................................................ 1 Chapter 2. Laboratory requirements and general techniques ......................... 19 Chapter 3. Tissue culture media ....................................................................... 39 Chapter 4. Cell culture ...................................................................................... 63 Chapter 5. Cellular totipotency ......................................................................... 95 Chapter 6. Somatic embryogenesis ................................................................... 125 Chapter 7. Haploid production .......................................................................... 167 Chapter 8. Triploid production .......................................................................... 215 Chapter 9. Variant selection .............................................................................. 231 Chapter 10. In vitro pollination and fertilization ............................................... 269 Chapter 11. Zygotic embryo culture .................................................................... 297 Chapter 12. Protoplast isolation and culture ..................................................... 337 Chapter 13. Somatic hybridization and cybridization ........................................ 373 Chapter 14. Genetic engineering ......................................................................... 407 Chapter 15. Production of pathogen-free plants ................................................. 451 Chapter 16. Clonal propagation .......................................................................... 483 Chapter 17. Production of secondary metabolites .............................................. 537 Chapter 18. Germplasm storage ......................................................................... 563 Glossary of terms commonly used in plant tissue culture .................................. 589 References ............................................................................................................. 603 Subject and plant index ........................................................................................ 749