Phospholipid signaling protocols

Phosphoinositidase C activation assay. 1, Cell labeling, stimulation, and recovery of cellular [³H]Phosphoinositides and [³H]phosphoinositols / Ian M. Bird -- Phosphoinositidase C activation assay. 2, Simple analysis of recovered cellular phosphoinositides and phosphoinositols / Ian M. Bird -- Phosphoinositidase C activation assay. 3, HPLC analysis of cellular phosphoinositides and phosphoinositols / Ian M. Bird -- Measurement of phosphoinositols and phosphoinositides using radio high-performance liquid chromatography flow detection / Lubo Zhang, Iain L. Buxton -- Preparation of [³H]phosphoinositol standards and conversion of [³H]phosphoinositides to [³H]phosphoinositols / Ian M. Bird -- Inositol 1,4,5-trisphosphate mass assay / Lubo Zhang -- Measurement of cellular diacylglycerol content / Wendy Bollinger Bollag, Richard D. Griner -- Phosphatidylinositol 4-kinases : assays and product analysis / Rachel E. Meyers, Lewis C. Cantley -- Phosphatidylinositol(3,4,5)trisphosphate (Ptdins(3,4,5)P₃) mass measurement using a radioligand displacement assay / Jeroen Kaay, Pete J. Cullen, C. Peter Downes -- Detection of phosphatidylinositol-4-phosphate 5-kinase activity using thin-layer chromatography / Gregory J. Parker, Joost C. Loijens, Richard A. Anderson -- Determination of phospholipase C-or phospholipase D-catalyzed phosphatidylcholine hydrolysis / Michael J. Wakelam, Trevor R. Pettitt -- Measurement of phospholipase D activity / Wendy Bollinger Bollag -- Monitoring of phospholipase A₂ activation in cultured cells using tritiated arachidonic acid / Sandie I. Briand, Sylvie G. Bernier, Gaétan Guillemette -- Assay of cellular diacylglycerol and monoacylglycerol lipases / Bryan A. Wolf -- Isotopic efflux studies as indices of phospholipase activation / Jacob Vadakekalam, Stewart Metz -- Extraction and measurements of prostanoids and leukotrienes by radioimmunoassays / Bruno Battistini, Serge Picard, Pierre Borgeat, Pierre Sirois -- Measurements of prostanoids, leukotrienes, and isoprostanes by enzyme immunoassays / Bruno Battistini, Serge Picard, Pierre Borgeat, Pierre Sirois -- Measurements of arachidonic acid metabolites derived from the lipoxygenase pathways by high-pressure liquid chromatography / Pierre Borgeat, Serge Picard, Bruno Battistini, Pierre Sirois -- Measurement of sphingomyelin and ceramide cellular levels after sphingomyelinase-mediated sphingomyelin hydrolysis / Pino Santana, Luisa F. Fanjul, C.M. Ruiz Galarreta -- Sphingomyelin and ceramide mass assay / Pino Santana, C.M. Ruiz Galarreta, Luisa F. Fanjul -- Sphingosine kinase : assay and product analysis / Ana Olivera, Sarah Spiegel -- Analytical methods and steps to sample preparation for determination of molecular species of fatty acids / Sanda Clejan -- HPLC analytical methods for the separation of molecular species of fatty acids in diacylglycerol and cellular phospholipids / Sanda Clejan -- Analysis of molecular species of cellular sphingomyelins and ceramides / Sanda Clejan -- Solubilization and assay of cellular and tissue protein / Cynthia E. Shaw -- Western immunoblot analysis / Cynthia E. Shaw, Jing Zheng -- Immunohistochemistry and immunocytochemistry / Jing Zheng -- Extraction of cellular and tissue RNA / Daniel S. Millican, Ian M. Bird -- Size separation and quantification of mRNA by Northern analysis / Ian M. Bird -- Preparation of single-stranded antisense cDNA probes by asymmetric PCR / Daniel S. Millican, Ian M. Bird -- Optimization of a reverse transcription-polymerase chain reaction (RT-PCR) mass assay for low-abundance mRNA / Jacqueline M. Cale, Cynthia E. Shaw, Ian M. Bird