DNA topoisomerase protocols. / Vol. 1, DNA topology and enzymes

Introduction to DNA topoisomerases / Mary-Ann Bjornsti, Neil Osheroff -- Resolution of DNA molecules by one-dimensional agarose-gel electrophoresis / Mary-Ann Bjornsti, Maureen D. Megonigal -- Two-dimensional agarose-gel electrophoresis of DNA topoisomers / Ryo Hanai, Joaquim Roca -- Analysis of altered DNA structures: cruciform DNA / Albert J. Courey -- Purification of supercoiled plasmid DNA / Kelly L. Jordan, Devon L. Evans, David J. Hall -- Purification and use of DNA minicircles with different linking numbers / Giorgio Camilloni, Rodolfo Negri, Micaela Caserta, Ernesto Mauro -- Isolation of kinetoplast DNA / Theresa A. Shapiro, Viiu A. Klein, Paul T. Englund -- Isolation of knotted DNA from coliphage P4 / Morten Isaksen, Bryan Julien, Richard Calendar, Björn H. Lindqvist -- Analysis of DNA knots and catenanes by agarose-gel electrophoresis / Stephen D. Levene, Hua Tsen -- Sedimentation analysis of bacterial nucleoid structure / Karl Drlica, Chang-Rung Chen, Samuel Kayman -- Coating DNA with RecA protein to distinguish DNA path by electron microscopy / E. Lynn Zechiedrich, Nancy J. Crisona -- Methods for analyzing DNA bending / Jason D. Kahn -- Formation of extrachromosomal DNA rings in Saccharomyces cerevisiae using site-specific recombination / Marc R. Gartenberg -- Overexpression and purification of bacterial DNA gyrase / Anthony Maxwell, Alison J. Howells -- Overexpression and purification of bacterial DNA topoisomerase I / Chang-Xi Zhu, Yuk-Ching Tse-Dinh -- Overexpression and purification of Escherichia coli DNA topoisomerase III / Russell J. DiGate -- Overexpression and purification of bacterial topoisomerase IV / Hong Peng, Kenneth J. Marians -- Purification of the bacteriophage T4 type II DNA topoisomerase / Kenneth N. Kreuzer, Sue H. Neece -- Overexpression and purification of DNA topoisomerase I from yeast / Mary-Ann Bjornsti, Jolanta Fertala -- Overexpression and purification of Saccharomyces cerevisiae DNA topoisomerase II from yeast / Janet E. Lindsley -- Purification of DNA topoisomerase II from Drosophila melanogaster / Stacie J. Froelich-Ammon, Paul S. Kingma, Neil Osheroff -- Purification of DNA topoisomerase I from human placenta / Joseph A. Holden -- Purification of baculovirus-expressed human DNA topoisomerase I / Lance Stewart, James J. Champoux -- Rapid purification of DNA topoisomerase II containing a hexahistidine tag by metal ion affinity chromatography / Harald Biersack, Sanne Jensen, Ole Westergaard -- Metabolic labeling, immunoprecipitation, and two-dimensional tryptic phosphopeptide mapping of human topoisomerase II / Nicholas J. Wells, Ian D. Hickson -- Immunoblot analysis and band depletion assays / Scott H. Kaufmann, Phyllis A. Svingen -- Visualization of DNA topoisomerases by electron microscopy / Piero Benedetti, Alessandra Silvestri -- Compendium of DNA topoisomerase sequences / Paul R. Caron