Immunochemical protocols. / Vol. 80

Production of polyclonal antisera / Jonathan A. Green, Margaret M. Manson -- Raising polyclonal antibodies using nitrocellulose-bound antigen / Monique Diano, André Le Bivic, Michel Hirn -- Production and characterization of antibodies against synthetic peptides / David C. Hancock, Gerard I. Evan -- Preparation and testing of monoclonal antibodies to recombinant proteins / Christopher J. Dean -- A simple hollow-fiber bioreactor for the "in-house" production of monoclonal antibodies / Margaret Goodall -- Screening of monoclonal antibodies using antigens labeled with acetylcholinesterase / Yveline Frobert, Jacques Grassi -- Synthesis of peptides for use as immunogens / David C. Hancock, Nicola J. Reilly, Gerard I. Evan -- Use of proteins blotted to polyvinylidene difluoride membranes as immunogens / Boguslaw Szewczyk, Donald F. Summers -- Purification of glycoproteins and their use as immunogens / Boguslaw Szewczyk, Donald F. Summers -- IgG purification / Mark Page, Robin Thorpe -- Purification of monoclonal antibodies / Mark Page, Robin Thorpe -- Production of bispecific and trispecific F(ab)2 and F(ab)3 antibody derivatives / Ruth R. French -- Preparation of cytotoxic antibody-toxin conjugates / Alan J. Cumber, Edward J. Wawrzynczak -- Immunoaffinity purification and quantification of antibody-toxin conjugates / Edward J. Wawrzynczak, Alan J. Cumber -- Competitive ELISA / Kittima Makarananda, Lucinda R. Weir, Gordon E. Neal -- Epitope mapping / Glenn E. Morris -- Coupling of antibodies with biotin / Rosaria P. Haugland, Wendy W. You -- Preparation of avidin conjugates / Rosaria P. Haugland, Mahesh K. Bhalgat -- Enhanced chemiluminescence immunoassay / Richard A. Stott -- Nonradioactive methods for visualization of protein blots / Michael Finney -- Colloidal gold staining and immunoprobing on the same Western blot / Denise Egger, Kurt Bienz -- Erasure of Western blots after autoradiographic or chemiluminescent detection / Scott H. Kaufmann, Udo Kellner -- Colloidal gold staining and immunodetection in 2D protein mapping / Anthony H. Schapira -- Enzyme-antienzyme method for immunohistochemistry / Michael G. Ormerod, Elizabeth Philp, Susanne F. Imrie -- Immunohistochemical detection of bromodeoxyuridine-labeled nuclei for in vivo cell kinetic studies / Jonathan A. Green, Richard E. Edwards, Margaret M. Manson -- Cell kinetic studies using a monoclonal antibody to bromodeoxyuridine / George D. Wilson -- Immunohistochemical detection of cells in the division cycle using antibodies to proliferating cell nuclear antigen (PCNA) / Richard E. Edwards, Jerry Styles -- Double label immunohistochemistry on tissue sections using alkaline phosphatase and peroxidase conjugates / Jonathan A. Green, Margaret M. Manson -- Preparation of colloidal gold probes / David A. Hughes, Julian E. Beesley -- Immunogold probes in light microscopy / David A. Hughes, Julian E. Beesley -- Immunogold probes in electron microscopy / David J. Ferguson, David A. Hughes, Julian E. Beesley -- Electron microscopic-silver enhancement for double labeling with antibodies raised in the same species / Kurt Bienz, Denise Egger -- Flow cytometric analysis of cell surface antigen density / R. Adrian Robins -- Multiple immunofluorescence analysis of cells using flow cytometry / R. Adrian Robins -- Flow cytometric methods of analyzing apoptotic cells / Anne E. Milner, J. Michaela Levens, Christopher D. Gregory -- Flow cytometric detection of proliferation-associated antigens, PCNA and Ki-67 / George D. Wilson -- Sorting of human peripheral blood T-cell subsets using immunomagnetic beads / Eddie C.Y. Wang -- Preparation and use of nonradioactive hybridization probes / Victor T.-W. Chan -- Cellular human and viral DNA detection by nonisotopic in situ hybridization / C.S. Herrington -- Biotinylated probes in colony hybridization / Michael J. Haas -- Nonisotopic in situ hybridization : gene mapping and cytogenetics / Bhupendra Bhatt, Tulin Sahinoglu, Cliff Stevens -- Expression of recombinant antibody fusion proteins in E. coli / Stephen M. Hobbs -- Expression of antibody fusion proteins in mammalian cells / Anita A. Hamilton, John R. Adair, Simon J. Forster -- Antibody screening of bacteriophage [lambda] gt-11 DNA expression libraries / Peter Jones -- Display of antibody chains on filamentous bacteriophage / Peter Jones -- Production of human Fab antibody fragments from phage display libraries / J. Mark Hexham -- Antibodies from phage display libraries as immunochemical reagents / Dario Neri, Alessandro Pini, Ahuva Nissim