Telomeres and telomerase : methods and protocols

Preface ................................................................................ v Contributors ........................................................................ ix 1 Introduction to Telomeres and Telomerase Michael C. Bibby .......................................................... 1 2 Detection of Chromosome Ends by Telomere FISH Harry Scherthan ......................................................... 13 3 Telomere Length Distribution: Digital Image Processing and Statistical Analysis Jean-Patrick Pommier and Laure Sabatier ............. 33 4 Analysis of Telomerase RNA Gene Expression by In Situ Hybridization W. Nicol Keith, Joseph Sarvesvaran, and Martin Downey............................................... 65 5 Relative Gene Expression in Normal and Tumor Tissue by Quantitative RT-PCR Dennis S. Salonga, Kathleen D. Danenberg, Jean Grem, Ji Min Park, and Peter V. Danenberg ....................................... 83 6 Quantitative Detection of Telomerase Components by Real-Time, Online RT-PCR Analysis with the LightCycler Thomas Emrich, Sheng-Yung Chang, Gerlinde Karl, Birgit Panzinger, and Chris Santini.................... 99 7 Standard TRAP Assay Angelika M. Burger .................................................. 109 8 Stretch PCR Assay Jun-ichi Nakayama and Fuyuki Ishikawa ............. 125 viii Contents 9 Fluorescent Detection of Telomerase Activity Wade K. Aldous, Amber J. Marean, Mary J. DeHart, and Katherine H. Moore ..................................... 137 10 Nonradioactive Detection of Telomerase Activity Using a PCR–ELISA-Based Telomeric Repeat Amplification Protocol Thomas Emrich and Gerlinde Karl ........................ 147 11 In Situ TRAP Assay Detection of Telomerase Activity in Cytological Preparations Kazuma Ohyashiki and Junko H. Ohyashiki ........ 159 12 Biotinylated Primer for Detecting Telomerase Activity Without Amplification Daekyu Sun ............................................................... 165 13 Whole-Cell and Microcell Fusion for the Identification of Natural Regulators of Telomerase Henriette Gourdeau, Marsha D. Speevak, Lucie Jetté, and Mario Chevrette ..................... 173 14 Screening with COMPARE Analysis for Telomerase Inhibitors Imad Naasani, Takao Yamori, and Takashi Tsuruo............................................ 197 15 Telomerase as a Therapeutic Target: Therapeutic Potential of Telomerase Inhibitors John A. Double ......................................................... 209 Index ................................................................................ 217