Embryonic stem cells : methods and protocols

Preface ............................................................................................................. v Contributors ..................................................................................................... xi Color Plates .................................................................................................... xv 1 Methods for the Isolation and Maintenance of Murine Embryonic Stem Cells Marsha L. Roach and John D. McNeish ............................................... 1 2 The Use of Chemically Defined Media for the Analyses of Early Development in ES Cells and Mouse Embryos Gabriele Proetzel and Michael V. Wiles .............................................17 3 Analysis of the Cell Cycle in Mouse Embryonic Stem Cells Pierre Savatier, Hélène Lapillonne, Ludmila Jirmanova, Luigi Vitelli, and Jacques Samarut ................................................. 27 4 Murine Embryonic Stem Cells as a Model for Stress Proteins and Apoptosis During Differentiation André-Patrick Arrigo and Patrick Mehlen ..........................................35 5 Effects of Altered Gene Expression on ES Cell Differentiation Yong Fan and J. Richard Chaillet ....................................................... 45 6 Hypoxic Gene Regulation in Differentiating ES Cells David M. Adelman and M. Celeste Simon ..........................................55 7 Regulation of Gap Junction Protein (Connexin) Genes and Function in Differentiating ES Cells Masahito Oyamada, Yumiko Oyamada, Tomoyuki Kaneko, and Tetsuro Takamatsu .................................................................... 63 8 Embryonic Stem Cell Differentiation as a Model to Study Hematopoietic and Endothelial Cell Development Stuart T. Fraser, Minetaro Ogawa, Satomi Nishikawa, and Shin-Ichi Nishikawa ................................................................... 71 9 Analysis of Bcr-Abl Function Using an In Vitro Embryonic Stem Cell Differentiation System Takumi Era, Stephane Wong, and Owen N. Witte............................. 83 10 Embryonic Stem Cells as a Model for Studying Osteoclast Lineage Development Toshiyuki Yamane, Takahiro Kunisada, and Shin-Ichi Hayashi ...... 97 11 Differentiation of Embryonic Stem Cells as a Model to Study Gene Function During the Development of Adipose Cells Christian Dani ......................................................................................107 12 Embryonic Stem Cell Differentiation and the Vascular Lineage Victoria L. Bautch ................................................................................117 13 Embryonic Stem Cells as a Model to Study Cardiac, Skeletal Muscle, and Vascular Smooth Muscle Cell Differentiation Anna M. Wobus, Kaomei Guan, Huang-Tian Yang, and Kenneth R. Boheler .................................................................127 14 Cardiomyocyte Enrichment in Differentiating ES Cell Cultures: Strategies and Applications Kishore B. S. Pasumarthi and Loren J. Field ................................. 157 15 Embryonic Stem Cells as a Model for the Physiological Analysis of the Cardiovascular System Jürgen Hescheler, Maria Wartenberg, Bernd K. Fleischmann, Kathrin Banach, Helmut Acker, and Heinrich Sauer ................. 169 16 Isolation of Lineage-Restricted Neural Precursors from Cultured ES Cells Tahmina Mujtaba and Mahendra S. Rao ..........................................189 17 Lineage Selection for Generation and Amplification of Neural Precursor Cells Meng Li .................................................................................................205 18 Selective Neural Induction from ES Cells by Stromal Cell-Derived Inducing Activity and Its Potential Therapeutic Application in Parkinson's Disease Hiroshi Kawasaki, Kenji Mizuseki, and Yoshiki Sasai ................... 217 19 Epidermal Lineage Tammy-Claire Troy and Kursad Turksen ......................................... 229 20 ES Cell Differentiation Into the Hair Follicle Lineage In Vitro Tammy-Claire Troy and Kursad Turksen ......................................... 255 21 Embryonic Stem Cells as a Model for Studying Melanocyte Development Toshiyuki Yamane, Shin-Ichi Hayashi, and Takahiro Kunisada... 261 22 Using Progenitor Cells and Gene Chips to Define Genetic Pathways S. Steven Potter, M. Todd Valerius, and Eric W. Brunskill............269 23 ES Cell-Mediated Conditional Transgenesis Marina Gertsenstein, Corrinne Lobe and Andras Nagy................. 285 24 Switching on Lineage Tracers Using Site-Specific Recombination Susan M. Dymecki, Carolyn I. Rodriguez, and Rajeshwar B. Awatramani ..................................................... 309 viii Contents 25 From ES Cells to Mice: The Gene Trap Approach Francesco Cecconi and Peter Gruss ................................................335 26 Functional Genomics by Gene-Trapping in Embryonic Stem Cells Thomas Floss and Wolfgang Wurst .................................................347 27 Phage-Displayed Antibodies to Detect Cell Markers Jun Lu and Steven R. Sloan ..............................................................381 28 Gene Transfer Using Targeted Filamentous Bacteriophage David Larocca, Kristen Jensen-Pergakes, Michael A. Burg, and Andrew Baird............................................................................393 29 Single-Cell PCR Methods for Studying Stem Cells and Progenitors Jane E. Aubin, Fina Liu, and G. Antonio Candeliere...................... 403 30 Nonradioactive Labeling and Detection of mRNAs Hybridized onto Nucleic Acid cDNA Arrays Thorsten Hoevel and Manfred Kubbies ...........................................417 31 Expression Profiling Using Quantitative Hybridization on Macroarrays Geneviève Piétu and Charles Decraene ...........................................425 32 Isolation of Antigen-Specific Intracellular Antibody Fragments as Single Chain Fv for Use in Mammalian Cells Eric Tse, Grace Chung, and Terence H. Rabbitts ...........................433 33 Detection and Visualization of Protein Interactions with Protein Fragment Complementation Assays Ingrid Remy, André Galarneau, and Stephen W. Michnick ........... 447 34 Direct Selection of cDNAs by Phage Display Reto Crameri, Gernot Achatz, Michael Weichel, and Claudio Rhyner ....................................................................... 461 35 Screening for Protein–Protein Interactions in the Yeast Two-Hybrid System in Embryonic Stem Cells R. Daniel Gietz and Robin A. Woods ................................................ 471 Index .............................................................................................................487