Molecular cloning : a laboratory manual
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Joseph Sambrook; David W Russell
- ناشر : Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press
- چاپ و سال / کشور: 2001
- شابک / ISBN : 9780879695774
Description
Vol. 1: Ch. 1. Plasmids and their usefulness in molecular cloning -- Ch. 2. Bacteriophage and its vectors -- Ch. 3. Working with bacteriophage M13 vectors -- Ch. 4. Working with high-capacity vectors -- Ch. 5. Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis -- Ch. 6. Preparation and analysis of eukaryotic genomic DNA -- Ch. 7. Extraction, purification, and analysis of mRNA from eukaryotic cells -- Vol. 2: Ch. 8. In Vitro amplification of DNA by the polymerase chain reaction -- Ch. 9. Preparation of radiolabeled DNA and RNA probes -- Ch. 10. Working with synthetic oligonucleotide probes -- Ch. 11. Preparation of cDNA libraries and gene identification -- Ch. 12. DNA sequencing -- Ch. 13. Mutagenesis -- Ch. 14. Screening expression libraries -- Vol. 3: Ch. 15. Expression of cloned genes in Escherichia coli -- Ch. 16. Introducing cloned genes into cultured mammalian cells -- Ch. 17. Analysis of gene expression in cultured mammalian cells -- Ch. 18. Protein interaction technologies.
