ELISA : theory and practice
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : J R Crowther
- ناشر : Totowa, N.J. : Humana Press
- چاپ و سال / کشور: 1995
- شابک / ISBN : 9780896032798
Description
Basic immunology -- Basic principles of ELISA -- Stages in ELISA -- Theoretical considerations -- Direct ELISA : titration of antigen and antibody -- Indirect ELISA -- Use of antibodies on solid-phase in capture ELISA -- Competitive ELISA -- Immunochemical techniques.
ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow you to fully appreciate exactly how and why assays function, as well as to efficiently develop assays suitable to your own research that are both rapid and accurate
