Signal transduction protocols

The problems and pitfalls of radioligand binding / Mary Keen -- Solubilization and purification of a functional ionotropic excitatory amino acid receptor / Jeremy M. Henley, David M. Kirkham -- Autoradiography of [beta]₁- and [beta]₂-adrenoceptors / Roger J. Summers, Peter Molenaar -- In situ hybridization / Brian J. Morris -- Measurement of the GTPase activity of signal-transducing G-proteins in neuronal membranes / Marva Sweeney -- Measurement of adenylyl cyclase activity in cell membranes / Robert J. Williams, Eamonn Kelly -- The measurement of cyclic AMP levels in biological preparations / Stephen P.H. Alexander -- Mass measurements of cyclic AMP formation by radioimmunoassay, enzyme immunoassay and scintillation proximity assay / Jeffrey K. Horton, Peter M. Baxendale -- Measurement of cyclic GMP formation / Felix Hernandez -- Cyclic AMP-dependent protein kinase activity ratio assay / Kenneth J. Murray -- Purification and activation of cyclic GMP-dependent protein kinase / Takayoshi Kuno, Hideyuki Mukai -- The analysis and assay of cyclic nucleotide phosphodiesterase isoenzyme activity / Mohammed Shahid, C. David Nicholson -- Separation of labeled inositol phosphate isomers by high-pressure liquid chromatography (HPLC) / Stephen Jenkinson -- Mass measurement of key phosphoinositide cycle intermediates / R.A. John Chaliss -- Measurement of phospholipase C activity in brain membranes / Enrique Claro, Elisabet Sarri, Fernando Picatoste -- Measurement of diacylglycerol : origins of receptor-stimulated diacylglycerol accumulation / Michael R. Boarder, John R. Purkiss -- Measurement of intracellular free calcium ion concentration in cell populations using fura-2 / Philip A. Iredale, John M. Dickenson -- Measurement of Ca²⁺ fluxes in permeabilized cells using ⁴⁵Ca²⁺ and fluo-3 / Robert A. Wilcox, James Strupish, Stefan R. Nahorski -- Single-cell calcium imaging / Katrina A. Marsh -- Characterization of calcium/calmodulin-stimulated protein kinase II / Paula E. Jarvie, Peter R. Dunkley -- Protein kinase C : measurement of translocation, activation and role in cellular responses / Sandra E. Wilkinson, Trevor J. Hallam -- The measurement of phospholipase D-linked signaling in cells / Michael J.O. Wakelam, Matthew Hodgkin, Ashley Martin -- Phospholipase A₂ activity / Ronald M. Burch -- Techniques for the measurement of nitric oxide / Anna M. Leone ... [et al.].