Protein NMR techniques
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : David G Reid
- ناشر : Totowa, N.J. : Humana Press
- چاپ و سال / کشور: 1997
- شابک / ISBN : 9780896033092
Description
Introduction to the NMR of proteins -- Double and triple resonance NMR methods for protein assignment -- Protein chemical shifts -- Data processing in multidimensional NMR -- Calculating protein structures from NMR data -- Studies of protein-ligand interactions by NMR -- NMR of paramagnetic proteins -- NMR studies of peptides and proteins associated with membranes -- Metal NMR for the study of metalloproteins -- Production and characterization of recombinant proteins for NMR structural studies -- A case history : NMR studies of the structure of a small protein, [omega]-conotoxin MVIIA -- Spectrometer calibration and experimental setup : basic principles and procedures
In Protein NMR Techniques leading experts demonstrate how to successfully apply the fundamentals of NMR to the study of proteins. They cover not only assignment and structure determination techniques, but also the practical aspects of experimental set-up, protein production, data processing, and binding studies. Extensive treatments of paramagnetics and metalloproteins, membrane-bound proteins, and protein-ligand complexes are also included
