YAC protocols

Generation of large insert YAC libraries / Zoia Larin, Anthony P. Monaco, Hans Lehrach -- YAC library storage and transport / John E. Collins, Sheila Hassock, Ian Dunham -- YAC library screening 1. Preparation of hybridization filters and PCR pools / Charlotte G. Cole, John E. Collins, Ian Dunham -- YAC library screening 2. Hybridization and PCR-based screening protocols / Charlotte G. Cole, John E. Collins, Ian Dunham -- Cloning of human telomeres in Saccharomyces cerevisiae / Gillian Bates -- Purification of YAC-containing total yeast DNA / Gary A. Silverman -- Restriction analysis of YACs / Jiannis Ragoussis -- RARE-cleavage analysis of YACs / Shawn P. Iadonato, Andreas Gnirke -- YAC localization by fluorescence in situ hybridization / Gabriele Senger -- Alu-PCR fingerprinting of YACs / Alison Coffey, Simon Gregory, Charlotte G. Cole -- Detection of chimerism in YAC clones / Sandro Banfi, Huda Y. Zoghbi -- Amplification with arbitrary primers / Anna Rienzo, Amy C. Peterson, Nelson B. Freimer -- End rescue from YACs using the vectorette / Donald J. Ogilvie, Louise A. James -- Isolation of YAC ends by plasmid rescue / Gillian Bates -- End-rescue of YAC clone inserts by inverse PCR / Gary A. Silverman -- Covering YAC-cloned DNA with phages and cosmids / Jiannis Ragoussis, Anthony P. Monaco -- Fragmentation and integrative modification of YACs / Jennifer W. McKee-Johnson, Roger H. Reeves -- Targeting mutations to YACs by homologous recombination / Karen Duff, Clare Huxley -- Reconstruction of large genomic segments of DNA by meiotic recombination between YACs / Gary A. Silverman -- Genomic reconstruction by mitotic recombination of YACs / David Markie, Jiannis Ragoussis -- Amplification of the copy number of YACs / Lucy L. Ling, Douglas R. Smith, Donald T. Moir -- Transfer of YAC clones to new yeast hosts / Forrest Spencer, Giora Simchen -- Use of ACEDB as a database for YAC library data management / Ian Dunham, Gareth Ll. Maslen -- YAC transfer into mammalian cells by cell fusion / Nicholas P. Davies, Clare Huxley -- YAC transfer by microinjection / Andreas Schedl, Brenda Grimes, Lluís Montoliu -- Transfection of mammalian cells via lipofection / William M. Strauss -- The isolation of cDNAs by hybridization of YACs to cDNA libraries / Russell G. Snell -- cDNA selection with YACs / Satish Parimoo -- Markers, selection, and media in YAC cloning / David Markie.