In vitro mutagenesis protocols

Site-directed mutagenesis using positive antibiotic selection / Richard N. Bohnsack -- In vitro site-directed mutagenesis using the unique restriction site elimination (USE) method / Li Zhu -- Site-directed mutagenesis using double-stranded plasmid DNA templates / Jeffrey Braman, Carol Papworth, Alan Greener -- Site-directed mutagenesis using a uracil-containing phagemid template / Christian Hagemeier -- Oligonucleotide-directed mutagenesis using an improved phosphorothioate approach / Susan J. Dale, Ian R. Felix -- Analysis of point mutations by use of amber stop codon suppression / Scott A. Lesley -- A simple method for site-directed mutagenesis with double-stranded plasmid DNA / Derhsing Lai, Sidney Pestka -- Double-stranded DNA site-directed mutagenesis / Stéphane Viville -- Solid-phase in vitro mutagenesis using a plasmid DNA template / Roy Edward -- Targeted mutagenesis mediated by the triple helix formation / Peter M. Glazer, Gun Wang, Pamela A. Havre, Edward J. Gunther -- A universal nested deletion method using an arbitrary primer and elimination of a unique restriction site / Li Zhu, Ann E. Holtz -- Ordered deletions using exonuclease III / Denise Clark, Steven Henikoff -- Ligase chain reaction for site-directed in vitro mutagenesis / Gerard J. Rouwendal, Emil J. Wolbert, Lute-Harm Zwiers, Jan Springer -- PCR-based site-directed mutagenesis / Atsushi Shimada -- In vitro recombination and mutagenesis by overlap extension PCR / Robert J. Pogulis, Abbe N. Vallejo, Larry R. Pease -- Site-directed mutagenesis using overlap extension PCR / Ashok Aiyar, Yan Xiang, Jonathan Leis -- Modification of the overlap extension method for extensive mutagenesis on the same template / Ivan Mikaelian, Alain Sergeant -- Site-directed mutagenesis in vitro by megaprimer PCR / Sailen Barik -- Using PCR for rapid site-specific mutagenesis in large plasmids / Brynmor A. Watkins, Marvin S. Reitz -- PCR-assisted mutagenesis for site-directed insertion/deletion of large DNA segments / Daniel C. Tessier, David Y. Thomas -- Site-directed mutagenesis using a rapid PCR-based method / Gina L. Costa, John C. Bauer, Barbara McGowan, Mila Angert, Michael P. Weiner -- A simple method to introduce internal deletions or mutations into any position of a target DNA sequence / Marjana Tomic-Canic, Françoise Bernerd, Miroslav Blumenberg -- A simple method for site-specific mutagenesis that leaves the rest of the template unaltered / Marjana Tomic-Canic, Ivana Sunjevaric, Miroslav Blumenberg -- Multiple site-directed mutagenesis / Kolari S. Bhat -- Construction of linker-scanning mutations by oligonucleotide ligation / Grace M. Hobson, Patricia P. Harlow, Pamela A. Benfield -- Construction of linker-scanning mutations using PCR / Patricia P. Harlow, Grace M. Hobson, Pamela A. Benfield -- Use of codon cassette mutagenesis for saturation mutagenesis / Deena M. Kegler-Ebo, Glenda W. Polack, Daniel DiMaio -- Saturation mutagenesis by mutagenic oligonucleotide-directed PCR amplification (Mod-PCR) / Lillian W. Chiang -- Random mutagenesis of short target DNA sequences via PCR with degenerate oligonucleotides / Frank Kirchhof, Ronald C. Desrosiers -- Random sequence mutagenesis for the generation of active enzymes / Margaret E. Black, Lawrence A. Loeb -- Random mutagenesis by using mixtures of dNTP and dITP in PCR / Oscar P. Kuipers -- PCR-mediated chemical mutagenesis / Donald J. Roufa -- Oligonucleotide-directed random mutagenesis using the phosphorothioate method / Susan J. Dale, Maxine Belfield -- An efficient random mutagenesis technique using an E. coli mutator strain / Alan Greener, Marie Callahan, Bruce Jerpseth.