Recombinant gene expression protocols

Recombinant gene expression protocols : overview of experimental strategies for the expression of recombinant genes / Rocky S. Tuan -- Designing expression plasmid vectors in E. coli / Paulina Balbás -- Design of bacterial hosts for lac-based expression vectors / Herbert P. Schweizer, RoxAnn R. Karkhoff-Schweizer -- Bacteriophage [lambda]-based expression vectors / Alan C. Christensen -- Strategies for gene fusions / Stefan Ståhl, Per-Åke Nygren, Mathias Uhlén -- Application of the E. coli trp promoter / Daniel G. Yansura, Steven H. Bass -- Expression of proteins in E. coli utilizing a dual promoter-based vector : pLACT7 / George A. Garcia, Shaorong Chong -- Expression and secretion of proteins in E. coli / Ophry Pines, Masayori Inouye -- Synthetic two-cistron expression system / Brigitte E. Schoner -- Expression of cDNA-encoded proteins by cell-free transcription and translation / Chistopher Coleclough -- Yeast plasmids / Keshav K. Singh, Jack A. Heinemann -- Inducible expression cassettes in yeast. GAL4 / Lawrence M. Mylin, James E. Hopper -- Inducible expression cassettes in yeast. ADH2 / Virginia L. Price -- Constitutive expression vectors : PGK / Ian R. Graham, Alistair Chambers -- Design and construction of recombinant vaccinia viruses / Christopher C. Broder, Patricia L. Earl -- A simplified method of screening for isolation of recombinant vaccinia virus / Haifeng Chen, R. Padmanabhan -- Design of retroviral expression vectors / Kenine E. Comstock, Nathaniel F. Watson, John C. Olsen -- Use of defective herpes-derived plasmid vectors / Filip Lim, Dean Hartley, Philip Starr, Song Song, Phung Lang, Linda Yu, Yaming Wang, Alfred I. Geller -- Use of baculovirus expression vectors / David R. O'Reilly -- Expression of exogenous genes in Xenopus oocytes, eggs, and embryos / Koichiro Shiokawa, Chie Koga, Yuzuru Ito, Mikihito Shibata -- Analysis of heterologous gene expression in Xenopus blastomeres / Sally A. Moody -- Overview of vector design for mammalian gene expression / Randal J. Kaufman -- Experimental strategies in efficient transfection of mammalian cells. Calcium phosphate and DEAE-Dextran / Gregory S. Pari, Wayne A. Keown -- Experimental strategies in efficient transfection of mammalian cells. Electroporation / Donald C. Chang -- Highly effective delivery of foreign DNA to adherent cells via Polybrene/DMSO-assisted gene transfer / Rémy A. Aubin, Michael Weinfeld, Marzieh Taghavi, Razmik Mirzayans, Malcolm C. Paterson -- Selection of transfected cells : magnetic affinity cell sorting / Raji Padmanabhan, Snorri S. Thorgeirsson, R. Padmanabhan -- Selection of transfected cells and coamplification of transfected genes / Susan E. Kane -- Optimization of growth, viability, and specific productivity for expression of recombinant proteins in mammalian cells / Jennie P. Mather, Alison Moore, Robert Shawley -- Promoters to express cloned genes uniformly in Drosophila / Jean-Paul Vincent, Charles Girdham -- Application of micromechanical piercing structures for genetic transformation of nematodes / Sarwar Hashmi, Randy Gaugler -- Strategies in generating transgenic mammals / Olena Jacenko -- Use of gonadal primordial germ cells (PGCs) as tools for gene transfer in chickens / Nathalie Allioli, Gérard Verdier, Catherine Legras -- Strategies for the production of transgenic chickens / Robert J. Etches, Ann M. Verrinder Gibbins -- Plant cell transfection by electroporation / William B. Terzaghi, Anthony R. Cashmore -- Transformation and foreign gene expression in plants mediated by microprojectile bombardment / Henry Daniell -- Transformation of the cereals using agrobacterium / Jean Gould -- Transformation of microalgae using silicon carbide whiskers / Terri G. Dunahay, Sally A. Adler, Jonathan W. Jarvik