Protein sequencing protocols

Strategies for handling polypeptides on a microscale / Bryan John Smith, Paul Tempst -- SDS polyacrylamide gel electrophoresis for N-terminal protein sequencing / Bryan John Smith -- Two-dimensional polyacrylamide gel electrophoresis for the separation of proteins for chemical characterization / Michael J. Dunn -- Electroblotting of proteins from polyacrylamide gels for chemical characterization / Michael J. Dunn -- Enzymatic cleavage of polypeptides / Bryan John Smith, Colin Wheeler -- Chemical cleavage of polypeptides / Bryan John Smith -- Preparation of glycopeptides / Chris W. Sutton, Jacqui A. O'Neill -- Selective isolation of the carboxy terminal peptide from a protein / Tomoko Hayashi Akiyama, Tatsuru Sasagawa -- Selective isolation of the amino-terminal peptide from [alpha]-amino blocked protein / Tomoko Hayashi Akiyama, Tatsuru Sasagawa -- Analytical and micropreparative capillary electrophoresis of peptides / Alan J. Smith -- Reverse-phase HPLC purification of peptides from natural sources for structural analysis / Chris Shaw -- High-performance liquid chromatography on-line derivative spectroscopy for the characterization of peptides with aromatic amino acid residues / Christoph W. Turck -- Hydrolysis of samples for amino acid analysis / Ian Davidson -- Amino acid analysis : precolumn derivatization methods / G. Brent Irvine -- Postcolumn amino acid analysis / Alan J. Smith -- Fast-atom bombardment and electrospray mass spectrometry of peptides, proteins, and glycoproteins / Fiona M. Greer, Howard R. Morris -- Peptide mass fingerprinting using MALDI-TOF mass spectrometry / Darryl J. Pappin -- N-terminal protein ladder sequencing / Rong Wang, Brian T. Chait -- The Dansyl-Edman method for manual peptide sequencing / John M. Walker -- The Dansyl method for identifying N-terminal amino acids / John M. Walker -- Identification of PTH-amino acids by high-performance liquid chromatography / Gregory A. Grant, Mark W. Crankshaw -- Protein sequencer maintenance and troubleshooting / Bryan Dunbar -- Identifying the carboxy terminal amino acid of a protein / Tomoko Hayashi Akiyama, Tatsuru Sasagawa -- Enzymatic and chemical methods for manual C-terminal peptide sequencing / Franca Casagranda, John F. Wilshire -- Automated methods for C-terminal protein sequencing / Jerome M. Bailey, Chad G. Miller -- Chemical modification of proteins for sequence analysis / Alex F. Carne, Sally U -- On-membrane deblocking of proteins / Hisashi Hirano, Setsuko Komatsu, Susumu Tsunasawa -- Analysis of sites of protein phosphorylation / Alastair Aitken, Michèle Learmonth -- Identification of sites of glycosylation / Chris W. Sutton, Jacqui A. O'Neill -- Quantification and location of disulfide bonds in proteins / Alastair Aitken, Michèle Learmonth.