Ribozyme protocols

Ribozymes : an introduction / Helen A. James, Philip C. Turner -- Computer-aided calculation of the local folding potential of target RNA and its use for ribozyme design / Georg Sczakiel, Martin Tabler -- Computational approaches to the identification of ribozyme target sites / William James, Elizabeth Cowe -- Computer analysis of the conservation and uniqueness of ribozyme-targeted HIV sequences / Mary Beth Young, Arnold Hampel -- Selection of accessible sites for ribozymes on large RNA transcripts / Bruce L. Frank, John Goodchild -- Selection of efficient ribozyme cleavage sites in target RNAs / Andre Lieber, Mikkel Rohde, Michael Strauss -- Chemical synthesis, analysis, and purification of ribozymes / Ravi Vinayak -- A practical method for the production of RNA and ribozymes / Francine E. Wincott, Nassim Usman -- Preparation of templates for production of ribozymes and substrates / Rajesh K. Gaur, Guido Krupp -- Enzymatic synthesis and characterization of unmodified ribozymes and substrates / Guido Krupp -- T7 transcript length determination using enzymatic RNA sequencing / Andrew Siwkowski -- Chemical and enzymatic approaches to construct modified RNAs / Rajesh K. Gaur, Guido Krupp -- Applications of modified transcripts / Rajesh K. Gaur, Frank Conrad, Guido Krupp -- Cloning strategies for catalytic antisense RNAs / Martin Tabler, Mina Tsagris -- PCR-based construction of long hammerhead ribozymes / Martin Zillmann, Gregory Robinson -- Design and production of asymmetric hammerhead ribozymes / Martin Tabler, Georg Sczakiel -- Minimized hammerhead ribozymes / Maxine J. McCall, Philip Hendry, Trevor J. Lockett -- Design of hairpin ribozymes for in vitro and cellular applications / Qiao Yu, John M. Burke -- Design of the hairpin ribozyme for targeting specific RNA sequences / Arnold Hampel, Mary Beth Young, Scott Galasinski, Andrew Siwkowski -- Design and preparation of sequence-specific RNase P ribozymes / Denis Drainas, Guido Krupp -- Theoretical considerations in measuring reaction parameters / Timothy S. McConnell -- Experimental approaches for measuring reaction parameters / Timothy S. McConnell -- Determination of catalytic parameters for hairpin ribozymes / Mary Beth Young, Andrew Siwkowski, Arnold Hampel -- Characterizing ribozyme cleavage reactions / Philip Hendry, Maxine J. McCall, Trevor J. Lockett -- Defining optimum reaction conditions for hammerhead ribozymes / Philip Hendry, Maxine J. McCall, Trevor J. Lockett -- Using fluorescence resonance energy transfer to investigate hammerhead ribozyme kinetics / Thomas A. Perkins, John Goodchild -- Design of hybridizing arms in hammerhead ribozymes / Philip Hendry, Maxine J. McCall, Trevor J. Lockett -- Optimization of hammerhead flanking sequences using oligonucleotide facilitators / John Goodchild -- Enhancement of ribozyme function by RNA binding proteins / Nan Sook Lee, Edouard Bertrand, John J. Rossi -- Selection of fast-hybridizing complementary RNA species in vitro / Ralf Kronenwett, Georg Sczakiel -- In vitro selection of hairpin ribozymes / Bruno Sargueil, John M. Burke -- The detection of hammerhead ribozyme cleavage by RT-PCR methods / Rhonda Perriman -- Detection of ribozyme cleavage products using reverse ligation-mediated PCR (RL-PCR) / Edouard Bertrand, Micheline Fromont-Racine, Raymond Pictet, Thierry Grange -- Quantitation of ribozyme target abundance by QCPCR / Amber A. Beaudry, James A. McSwiggen -- Trans-splicing reactions by ribozymes / Joshua T. Jones, Seong-Wook Lee, Bruce A. Sullenger -- Ligation of RNA molecules by the hairpin ribozyme / Alfredo Bertal-Herranz, John M. Burke -- Mutagenesis and modeling of the hairpin ribozyme family / Andrew Siwkowski, Mary Beth DeYoung, Pamela Anderson, Arnold Hampel -- Preparation of homogeneous ribozyme RNA for crystallization / Jennifer A. Doudna -- Establishing suitability of RNA preparations for crystallization : determination of polydispersity / Adrian R. Ferré-d'Amaré, Jennifer A. Doudna -- A sparse matrix approach to crystallizing ribozymes and RNA motifs / Jamie H. Cate, Jennifer A. Doudna -- Crystallographic analyses of chemically synthesized modified hammerhead RNA sequences as a general approach toward understanding ribozyme structure and function / William G. Scott -- tRNA delivery systems for ribozymes / Rhonda Perriman, Rob de Feyter -- Expressing ribozymes in plants / Rob de Feyter, Judith Gaudron -- Using microinjection of Xenopus oocytes to express and optimize ribozymes in vivo / Philip C. Turner -- Exogenous cellular delivery of ribozymes and ribozyme encoding DNAs / Daniela Castanotto, Edouard Bertrand, John Rossi -- Optimization of lipid-mediated ribozyme delivery to cells in culture / Suzy A. Brown, Thale C. Jarvis -- Retroviral delivery and anti-HIV testing of hammerhead ribozymes / Laurence Cagnon, John Rossi -- Hairpin ribozyme gene therapy for AIDS / Elizabeth A. Duarte, Mark C. Leavitt, Osamu Yamada, Mang Yu -- Clinical aspects of ribozymes as therapeutics in gene therapy / David Looney, Mang Yu