Neuropeptide protocols

Preparation of neuropeptide-containing fractions from biological materials / J. Michael Conlon -- Purification of extracted peptides for structural analysis / Chris Shaw -- Amino acid sequencing of neuropeptides / Ka Wan Li, Wijnand P. Geraerts -- Neuropeptide gene identification using the polymerase chain reaction / Aaron G. Maule, Timothy G. Geary -- Solid-phase synthesis of neuropeptides by fmoc strategies / Chris Kowalczyk, Michael O'Shea -- Incorporation of stable pseudopeptide bonds : methylene amino, thioether, and hydroxyethylene derivatives / Graeme J. Anderson -- Synthesis of conformationally restricted peptides / Annette G. Beck-Sickinger -- Purification of synthetic peptides by high performance liquid chromatography / D. David Smith, Ann M. Hanly -- Molecular weight estimation for neuropeptides using size-exclusion high performance liquid chromatography / G. Brent Irvine -- Molecular weight determinations using polyacrylamide gel electrophoresis with tris-tricine buffers / G. Brian Wisdom -- Determination of neuropeptides by capillary electrophoresis / SungAe S. Park, Wei-Lun Hung, Daniel E. Schaufelberger, Norberto A. Guzman, Juan P. Advis -- Characterization of neuropeptide processing by fast atom bombardment mass spectrometry / Jerzy Silberring -- Analysis of neuropeptides by size-exclusion HPLC linked to electrospray ionization mass spectrometry / Jerzy Silberring -- Identification of peptides by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) and direct analysis of the laterobuccal nerve from the pond snail lymnaea stagnalis / Glenn Critchley, Belinda Worster -- Use of circular dichroism to determine secondary structure of neuropeptides / Laszlo Otvos -- ¹H nuclear magnetic resonance (NMR) in the elucidation of peptide structure / David J. Guthrie -- The study of membrane- or receptor-bound neuropeptides by NMR / Rickey P. Hicks -- Molecular modeling of neuropeptides / Sándor Lovas, Richard F. Murphy -- Tritium labeling of neuropeptides / Géza Tóth, Sándor Lovas, Ferenc Ötvös -- The use of IODO-GEN for preparing ¹²⁵I-labeled peptides and their purification by reversed-phase high performance liquid chromatography / J. Michael Conlon -- Production of antisera using peptide conjugates / Thomas E. Adrian -- Radioimmunoassay / Thomas E. Adrian -- Enzyme-linked immunosorbent assay of peptides / Laszlo Otvos, Gyorgyi I. Szendrei -- Sample preparation for peptide immunocytochemistry / Kathy M. Pogue, Colin F. Johnston -- Immunocytochemical methods for regulatory peptides / Kathy M. Pogue, Colin F. Johnston -- Ultrastructural localization of peptides using immunogold labeling / David W. Halton, Gerard P. Brennan -- Preparation of a membrane fraction for receptor studies and solubilization of receptor proteins with retention of biological activity / Mark Wheatley, John Howl, Nicola J. Yarwood, Andrew R. Davies, Rosemary A. Parslow -- Radioligand binding using ¹²⁵I-labeled peptides / Peter W. Abel, David Waugh, William B. Jeffries -- Analysis of data from "cold saturation" radioligand binding experiments / William B. Jeffries, David Waugh, Peter W. Abel -- Organ/tissue preparations for the assessment of agonist/antagonist activity / Mark Murnin, Sándor Lovas, James M. Allen, Richard F. Murphy -- Measurement of efflux rates from brain to blood / William A. Banks, Melita B. Fasold, Abba J. Kastin -- Assay of neuropeptidases using fluorogenic substrates / Carvell H. Williams -- Characterization of neuropeptidases using inhibitors / Nigel M. Hooper