Recombinant protein protocols : detection and isolation

Overview of experimental strategies on the detection and isolation of recombinant proteins and their applications / Rocky S. Tuan -- Reporter systems / Debyra Groskreutz, Elaine T. Schenborn -- Detection of recombinant protein based on reporter enzyme activity : chloramphenicol acetyltransferase / Peiyu Lee, Dennis E. Hruby -- Human placental alkaline phosphatase as a marker for gene expression / Paul Bates, Michael H. Malim -- Use of secreted alkaline phosphatase as a reporter of gene expression in mammalian cells / Steven R. Kain -- Detection of [beta]-galactosidase and [beta]-glucuronidase using chemiluminescent reporter gene assays / Corinne E. Olesen, John J. Fortin, John C. Voyta, Irena Bronstein -- Chemiluminescent immunoassay for the detection of chloramphenicol acetyltransferase and human growth hormone reporter proteins / Corinne E. Olesen, John C. Voyta, Irena Bronstein -- Detection and selection of cultured cells secreting recombinant product by soft agar cloning and antibody overlay / Marylou G. Gibson, Karmen Hodges, Lauretta Lowther -- Detection and isolation of recombinant protein based on binding affinity reporter. Maltose binding protein / Paul Riggs -- Detection and isolation of recombinant proteins based on binding affinity of reporter. Protein A / Stefan Ståhl, Per-Åke Nygren, Mathias Uhlén -- Expression and purification of recombinant streptavidin-containing chimeric proteins / Takeshi Sano, Cassandra L. Smith, Charles R. Cantor -- Bacterial expression, purification, and potential use of His-tagged GAL4 fusion proteins / M. Lienhard Schmitz, Patrick A. Baeuerle -- Detection of expressed recombinant protein based on multidrug resistance : P-glycoprotein / Ursula A. Germann -- Detection and isolation of recombinant proteins from mammalian cells by immunoaffinity chromatography : p53 / Jamil Momand, Bahman Sepehrnia -- Yeast GAL4 two-hybrid system : a genetic system to identify proteins that interact with a target protein / Li Zhu -- Alternative yeast two-hybrid systems : the interaction trap and interaction mating / Erica A. Golemis, Vladimir Khazak -- Detection of heterologous Gs-coupled receptor activity in LLC-PK1 cells based on expression of urokinase-type plasminogen activator / Luigi Catanzariti, Brian A. Hemmings -- Histochemical and fluorochrome-based detection of [beta]-galactosidase / Ruth Sullivan, Cecilia W. Lo -- Combined, sequential in situ hybridization and immunohistochemistry on the same tissue section / Kenneth J. Shepley, Rocky S. Tuan -- Whole mount in situ hybridization to embryos and embryonic tissues / Ronald A. Conlon -- Whole-mount in situ hybridization for developing chick embryos using digoxygenin-labeled RNA probes / Christopher W. Hsu, Rocky S. Tuan -- Methods for double detection of gene expression : combined in situ hybridization and immunocytochemistry or histochemistry / Ronald A. Conlon -- In situ PCR : current protocol / Omar Bagasra, Muhammad Mukhtar, Farida Shaheen, Roger J. Pomerantz -- Expression and detection of green fluorescent protein (GFP) / Steven R. Kain, Paul Kitts -- Transgenic animals as bioreactors for expression of recombinant proteins / Jaspal S. Khillan -- Artificial cells for bioencapsulation of cells and genetically engineered E. coli : for cell therapy, gene therapy, and removal of urea and ammonia / Thomas M. Chang, Satya Prakash -- Hyperexpression of a synthetic protein-based polymer gene / Henry Daniell, Chittibabu Guda, David T. McPherson, Xiaorong Zhang, Jie Xu, Dan W. Urry -- Transplantation of cells in an immunoisolation device for gene therapy / Victoria E. Carr-Brendel, Robin L. Geller, Tracy J. Thomas, Daniel R. Boggs, Susan K. Young, JoAnne Crudele, Laura A. Martinson, David A. Maryanov, Robert C. Johnson, James H. Brauker -- Delivery of recombinant HIV-1-directed antisense and ribozyme genes / Georg Sczakiel, Giorgio Palú, William James -- Inducible system designed for future gene therapy / Yaolin Wang, Bert W. O'Malley, Sophia Y. Tsai -- Human gene therapy : dreams to realization / Muhammad Mukhtar, Zahida Parveen, Omar Bagasra