Basic DNA and RNA protocols

The simultaneous isolation of RNA and DNA from tissues and cultured cells / Frank Merante, Sandeep Raha, Juta K. Reed, Gerald Proteau -- Restriction endonuclease digestion of DNA / Duncan R. Smith -- Agarose gel electrophoresis / Duncan R. Smith -- Capillary blotting of agarose gels / Duncan R. Smith, David Murphy -- Random primed ³²P-labeling of DNA / Duncan R. Smith -- Hybridization and competition hybridization of Southern blots / Rosemary E. Kelsell -- Utilization of DNA probes with digoxigenin-modified nucleotides in Southern hybridizations / Tim Helentjaris, Tom McCreery -- The preparation of fluorescein-labeled nucleic acid probes and their detection using alkaline phosphatase-catalyzed dioxetane chemiluminescence / Martin Cunningham, Martin Harris -- Monitoring incorporation of fluorescein into nucleic acid probes using a rapid labeling assay / Martin Cunningham -- The preparation of horseradish peroxidase-labeled nucleic acid probes and their detection using enhanced chemiluminescence / Bronwen Harvey, Ian Durrant, Martin Cunningham -- 3'-end labeling of oligonucleotides with fluorescein-11-dUTP and enhanced chemiluminescent detection / Martin Cunningham, Ian Durrant, Bronwen Harvey -- The preparation of riboprobes / Dominique Belin -- Native polyacrylamide gel electrophoresis / Adrian J. Harwood -- Use of silver staining to detect nucleic acids / Lloyd G. Mitchell, Angelika Bodenteich, Carl R. Merril -- End-labeling of DNA fragments / Adrian J. Harwood -- Northern blot analysis / Robb Krumlauf -- RNA slot blotting / David Murphy -- The RNase protection assay / Dominique Belin -- Primer extension analysis of mRNA / Mark W. Leonard, Roger K. Patient -- S1 mapping using single-stranded DNA probes / Stéphane Viville, Roberto Mantovani -- Nonradioactive in situ hybridization for cells and tissues / Ian Durrant -- In vitro packaging of DNA / Jeremy W. Dale, Peter J. Greenaway -- Construction of mammalian genomic libraries using [lambda] replacement vectors / Alan N. Bateson, Jeffrey W. Pollard -- The production of double-stranded complementary DNA for use in making libraries / Steve Mayall, Jane Kirk -- Construction of cDNA libraries / Michael M. Burrell -- Screening [lambda] libraries / Janet C. Harwood, Adrian J. Harwood -- Subcloning strategies and protocols / Danielle Gioioso Taghian, Jac A. Nickoloff -- Purification of DNA fragments from agarose gels using glass beads / Etienne Joly -- Transformation of E. coli / Fiona M. Tomley -- Transformation of bacteria by electroporation / Lucy Drury -- Preparation of plasmid DNA using alkaline lysis / Etienne Joly -- The rapid boiling method for small-scale preparation of plasmid DNA / Adrian J. Harwood -- Plasmid preparations with diatomaceous earth / Laura Machesky -- Polymerase chain reaction / Beverly C. Delidow, John P. Lynch, John J. Peluso, Bruce A. White -- Inverse polymerase chain reaction / Daniel L. Hartl, Howard Ochman -- Polymerase chain reaction with degenerate oligonucleotide primers to clone gene family members / Gregory M. Preston -- Cloning PCR products using T-vectors / Michael K. Trower, Greg S. Elgar -- Direct radioactive labeling of polymerase chain reaction products / Tim McDaniel, Stephen J. Meltzer -- A rapid PCR-based colony screening protocol for cloned inserts / Michael K. Trower -- Use of polymerase chain reaction to screen phage libraries / Lei Yu, Laura J. Bloem -- Cloning into M13 bacteriophage vectors / Qingzhong Yu -- Ordered deletions using exonuclease III / Denise Clark, Steven Henikoff -- M13 phage growth and single-stranded DNA preparation / Fiona M. Tomley -- Preparation of ssDNA from phagemid vectors / Michael K. Trower -- A rapid plasmid purification method for dideoxy sequencing / Annette M. Grifln, Hugh G. Grifln -- DNA sequencing using sequenase version 2.0 T7 DNA polymerase / Carl W. Fuller, Bernard F. McArdle, Annette M. Griffin, Hugh G. Griffin -- Pouring linear and buffer-gradient sequencing gels / Paul Littlebury -- Electrophoresis of sequence reaction samples / Alan T. Bankier -- Direct sequencing of PCR products / Janet C. Harwood, Geraldine A. Phear -- Thermal cycle dideoxy DNA sequencing / Barton E. Slatko -- Using the automated DNA sequencer / Zijin Du, Richard K. Wilson -- Terminal labeling of DNA for Maxam and Gilbert sequencing / Eran Pichersky -- DNA sequencing by the chemical method / Eran Pichersky -- Site-directed mutagenesis of double-stranded plasmids, domain substitution, and marker rescue by comutagenesis of restriction enzyme sites / Jac A. Nickoloff, Win-Ping Deng, Elizabeth M. Miller, F. Andrew Ray -- A protocol for site-directed mutagenesis employing a uracil-containing phagemid template / Michael K. Trower -- In vitro translation of messenger RNA in a rabbit reticulocyte lysate cell-free system / Louise Olliver, Charles D. Boyd -- In vitro translation of messenger RNA in a wheat germ extract cell-free system / Louise Olliver, Anne Grobler-Rabie, Charles D. Boyd -- One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin / Joanne Crowe, Brigitte Steude Masone, Joachim Ribbe