Receptor signal transduction protocols

Radioligand-binding methods for membrane preparations and intact cells / Mary Keen -- Site-directed mutagenesis and chimeric receptors in the study of receptor-ligand binding / Mark E. Olah, Gary L. Stiles -- Approaches to the stable transfection of G protein-coupled receptors / Andrea Townsend-Nicholson -- Methods for transient expression of hetero-oligomeric ligand-gated ion channels / Hartmut Lüddens, Esa R. Korpi -- The generation of receptor-selective antibodies / John J. Mackrill -- In situ hybridization / Judith C. Mak, Peter J. Barnes -- Lmmunocytochemical methods for investigating receptor localization / Maria I. Fonseca, R. Dale Brown -- Measurement of agonist-stimulated [³⁵S]GTP[gamma]S binding to cell membranes / Sebastian Lazareno -- Autoradiographic visualization in brain of receptor-G protein coupling using [³⁵S]GTP[gamma]S binding / Laura J. Sim, Dana E. Selley, Steven R. Childers -- Agonist-induced high-affinity GTP hydrolysis as an index of receptor-mediated G protein activation in mammalian brain membranes / Yuji Odagaki, Kjell Fuxe -- Use of random-saturation mutagenesis to study receptor-G protein coupling / Ethan S. Burstein, Tracy A. Spalding, Mark R. Brann -- Identification and quantitation of G protein [alpha]-subunits / Ian Mullaney, Graeme Milligan -- Covalent modification of G proteins by affinity labeling / Martin Hohenegger, Michael Freissmuth, Christian Nanoff -- Heterologous expression of receptors and signaling proteins in adult mammalian sympathetic neurons by microinjection / Stephen R. Ikeda -- Nuclear application of antisense oligonucleotides by microinjection and ballistomagnetic transfer to identify G protein heterotrimers activating phospholipase C / Frank Kalkbrenner, Edgar Dippel, Matthias Schroff, Burghardt Wittig, Günter Schultz -- Use of antisense-generating plasmids to probe the function of signal transduction proteins in primary neurons / Fe C. Abogadie, Yvonne Vallis, Noel J. Buckley, Malcolm P. Caulfield -- Protocols employed in the investigation of G protein-coupled receptor phosphorylation / Andrew G. Tobin -- Assay of G protein-coupled receptor kinase activity by rhodopsin phosphorylation / Alison W. Gagnon, Eamonn Kelly -- Radioligand binding measurement of receptor sequestration in intact and permeabilized cells / Diana M. Slowiejko, Stephen K. Fisher -- Regulation of receptor expression : analysis of receptor mRNA and gene transcription / Philip A. Iredale, Maura E. Charlton, John D. Alvaro, Ronald S. Duman