Protein targeting protocols

Surface plasmon resonance : measuring protein interactions in real time / George Panayotou -- Microcalorimetry of protein-protein interactions / Alan Cooper -- Partitioning of proteins in Triton X-114 / James G. Pryde -- Co-immunoprecipitation : identification of interacting proteins / Neil G. Anderson -- Overlay, ligand blotting, and band-shift techniques to study kinase anchoring / Zachary E. Hausken, Vincent M. Coghlan, John D. Scott -- Size-exclusion chromatography : identification of interacting proteins / James Beattie -- Overlay and bead assay : determination of calcium channel subunit interaction domains / Victoria E. Scott, Christina A. Gurnett, Kevin P. Campbell -- Use of GST-fusion and related constructs for the identification of interacting proteins / Mark Harris -- Molecular genetic approaches. 1, Two-hybrid systems / Graeme B. Bolger -- Molecular genetic approaches. 2, Expression-interaction cloning / Michael Csukai, Daria Mochly-Rosen -- Molecular genetic approaches. 3, Determination of protein sequence motifs involved in protein targeting by use of coupled transcription-translation systems / Grant Scotland, Miles D. Houslay -- Molecular genetic approaches. 4, Recombinant expression of wild-type and acylation-resistant G-protein [alpha]-subunits using transient transfection systems / Morag A. Grassie, Graeme Milligan -- Use of synthetic peptides in the dissection of protein-targeting interactions / John D. Scott, Maree C. Faux -- SH2 and SH3 domains : unraveling signaling networks with peptide antagonists / Rob Stein -- PH domain of serine-threonine protein kinase B (RAC-PKB) : expression and binding assay for phosphoinositides and inositol phosphates / Matthias Frech, Brian A. Hemmings -- Membrane targeting via protein N-myristoylation / R. A. McIlhinney -- Membrane targeting via protein palmitoylation / Michael Veit, Michael F. Schmidt -- Agonist-mediated turnover of G-protein [alpha]-subunit palmitoyl groups : role in membrane insertion / Morag A. Grassie, Graeme Milligan -- Membrane targeting via protein prenylation / Jean H. Overmeyer, Robert A. Erdman, William A. Maltese -- Nuclear protein import in a permeabilized cell assay / Frauke Melchior -- Clathrin-coated vesicles and targeting : preparation of adaptor proteins / Alain Pauloin -- Analysis of the sorting of secretory proteins to the regulated secretory pathway : a subcellular fractionation approach / Eric Chanat, Andrea S. Dittié, Sharon A. Tooze. --