Nitric Oxide Protocols

Enzymology of nitric oxide synthases / Benjamin Hemmens and Bernd Mayer -- Purification of the inducible nitric oxide synthase ; Purification of the constitutive nitric oxide synthase / Edward P. Garvey -- Cloning and expression of human inducible nitric oxide synthase / Mark L. Johnson, Richard A. Shapiro, Timothy R. Billiar -- Cloning and expression of human eNOS and nNos using the baculovirus-insect cell system / Ian G. Charles, Neale Foxwell, Ann Chubb -- Assay of NOS activity by the measurement of conversion of oxyhemoglobin to methemoglobin by NO / Mark Salter and Richard G. Knowles -- Measurement of NOS activity by conversion of radiolabeled arginine to citrulline using ion-exchange separation / Richard G. Knowles and Mark Salter -- Radiochemical measurement of NOS activity by conversion of [¹⁴C]L-arginine to citrulline using HPLC separation / James M. Cunningham and Richard C. Rayne -- The enzymatic measurement of nitrate and nitrite / Michael A. Titheradge -- Determination of NOS activity using cyclic-GMP formation / Suzanne G. Laychock -- Determination of NO with a Clark-type electrode / Kurt Schmidt and Bernd Mayer -- The measurement of NO in biological systems using chemiluminescence / Evangelos D. Michelakis and Stephen L. Archer -- Measurement of NO using electron paramagnetic resonance / S. Tsuyoshi Ohnishi -- Measurement of eNOS and iNOS mRNA expression using reverse transcription polymerase chain reaction / Norbert Reiling, Artur J. Ulmer, Sunna Hauschildt -- Measurement of NOS mRNA by Northern blotting and the ribonuclease-protection assay / Paloma Martín-Sanz and Lisardo Boscá -- Detection of NOS isoforms by Western-blot analysis / Fiona S. Smith and Michael A. Titheradge --Immunohistochemical localization of NOS isoforms / Victoria Cattell and Karen Mosley -- A practical protocol for the demonstration of NOS using in-situ hybridization / G. Michael Taylor and H. Terence Cook -- Use of NO donors in biological systems / Jayne M. Tullett and Daryl D. Rees -- Making and working with peroxynitrite / Roger White ... [et al.] -- The use of NO gas in biological systems / Neil Hogg and B. Kalyanaraman -- A microtiter-plate assay of human NOS isoforms / John Dawson and Richard G. Knowles -- Use of arginine analogs as inhibitors of nitric oxide synthase in rat-aortic rings / Rachel J. Russell -- Measurement of biopterin and the use of inhibitors of biopterin biosynthesis / Kazuyuki Hatakeyama -- A sensitive assay for the enzymatic activity of GTP cyclohydrolase I / Kazuyuki Hatakeyama and Toshie Yoneyama -- Simultaneous measurement of mitochondrial function and NO / Ignacio Lizasoain and María A. Moro -- Release of NO from donor compounds : a mathematical model for calculation of NO concentrations in the presence of oxygen / Kurt Schmidt ... [et al.] -- The determination of nitrotyrosine residues in proteins / Andrew J. Gow ... [et al.] -- Measurement of DNA damage using the comet assay / Steven Thomas ... [et al.] -- Methods for the study of NO-induced apoptosis in cultured cells / Anne C. Loweth and Noel G. Morgan.