Environmental monitoring of bacteria
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Clive Edwards
- ناشر : Totowa, N.J. : Humana Press,
- چاپ و سال / کشور: 1999
- شابک / ISBN : 9780896035669
Description
Some problems posed by natural environments for monitoring microorganisms -- Sampling sediment and soil: use of coring devices -- Sampling water bodies: tangential flow filtration -- Dielectrophoresis - Flow cytometry and cell sorting: rapid analysis and separation of individual bacterial cells from natural environments -- Magnetic particle-based separation techniques for monitoring bacteria from natural environments -- DNA extraction from natural environments -- Automated sequencing of DNA retrieved from environmental samples -- Analysis of DNA sequences -- Fluorescent polymerase chanin reaction/restriction fragment length polymorphism monitoring of genes amplified directly from bacterial communities in soils and sediments -- Recovery and analysis of ribosomal RNA sequences from the environment -- Application of denaturing gradient gel electrophoresis to microbial ecology -- Reporter gene expression for monitoring microorganisms -- Characterizing microorganisms in the environment by fatty acid analysis -- Fluorescent in situ hybridization and the analysis of the single cell -- Specific detection, viability assessment, and macromolecular staining of bacteria for flow cytometry -- Confocal laser scanning microscopy of environmental samples -- Monitoring microbial activities using membrane inlet mass spectrometry -- Experimental biofilms and their applications in the study of environmental processes -- Establishment of experimental biofilms using the modified robbins device and flow cells.
