Integrin protocols
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Anthony Howlett
- ناشر : Totowa, N.J. : Humana Press
- چاپ و سال / کشور: 1999
- شابک / ISBN : 9780896035690
Description
pt. 1. Working with integrins -- Integrin-binding peptides derived from phage display libraries -- Production of rat monoclonal antibiodies specific for mouse integrins -- Biochemical analysis of integrin-mediated Shc signaling -- Assays for kinase activity -- Immunoblotting of integrins -- Application of flow cytometry in the analysis and sterile sorting of cell populations based on integrin expression -- Expression of integrin transcripts in human cancer cells -- pt. 2. Functional analysis of integrins -- Functional analysis of the [beta]₂ integrins -- expression of heterologous integrin genes -- Preparing a polyclonal antibody to mouse [beta]₁ integrin with function-blocking activity -- Integrin gene targeting -- Determination of positive and negative regulatory elements -- pt. 3. Integrin function at the cellular level -- A cell-based adhesion assay for the characterization of integrin [alpha]v[beta]₃ antagonists -- Integrins in cell migration -- Models for studying cellular invasion of basement membranes -- Methods for studying anoikis -- Use of the 10-day-old chick embryo model for studying angiogenesis -- Cell shape and integrin signaling regulate the differentiation state of mammary epithelial cells
