In situ hybridization protocols
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Ian A Darby
- ناشر : Totowa, N.J. : Humana Press
- چاپ و سال / کشور: 2000
- شابک / ISBN : 9780896036864
Description
pt. 1. Chromosomal techniques -- Preparation of human partial chromosome paints from somatic cell hybirds -- DNA-protein In Situ convisualization for chromosome analysis -- Radioactive In Situ hybridization to animal chromosomes -- Native polytene chromosomes of Drosophila melanogaster for light and electron microscopic observation of the conformation and distribution of molecules -- Released chromatin or DNA fiber perparations for high-resolution fiber FISH -- In Situ hybridization to polytene chromosomes of Drosophila melanogaster and other dipteran species -- In Situ hybridization to polytene chromosomes -- Comparative gene mapping in exotic species using FISH -- pt. 2. Tissue techniques -- In Situ hybridization with nonradiaoactive probes -- Quantitative In Situ hybridization using radioactive probes to study gene expression in heterocellular systems -- In Situ end-labeling of fragmented DNA and the localization of apoptosis -- Combined immunohistochemical labeling and In Situ hybridization to colocalize mRNA and protein in tissue sections -- In Situ hybridization protocols for detection of viral DNA using radioactive and nonradioactive DNA probes -- Signal amplification for DNA and mRNA -- In Situ localization of PCR-amplified DNA and cDNA -- Ultrastructural detection of nucleic acids on thin sections of tissue embedded in hydrophilic resin -- In Situ hybridization for electron microscopy -- In Situ hybridization of whole-mount embryos -- cRNA probes: comparison of isotopic and nonisotopic detection methods -- pt. 3. Applications -- Molecular cytogenetic analysis of sperm from infertile males undergoing intracytoplasmic sperm injection -- Localization of HIV-1 DNA and tumor necrosis factor-[alpha] mRNA in human brain using polymerase chain reaction In Situ hybridization and immunocytochemistry
