Chemokine protocols
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Amanda E I Proudfoot; Timothy N C Wells; Christine Power
- ناشر : Totowa, N.J. : Humana Press
- چاپ و سال / کشور: 2000
- شابک / ISBN : 9780896037229
Description
Purification of chemokines from natural sources -- Cloning of novel chemokines using a signal sequence trap method -- Chemokine expression in insect cells -- Expression of chemokines in Escherichia coli -- Experession of chemokines in the periplasmic space of E. coli -- Synthesis of chemokines -- Identifcation of novel chemokines from expressed sequence of tag databases -- Purification of recombinant chemokines from E.coli -- Chemokine receptor cloning -- Generatin of stable cell lines expressing chemokine receptors -- Modified microchemotaxis assays -- Transwell chemotaxis -- Endothelial cell chemotaxis assays -- Radiolabeled chemokine binding assays -- Scintillation proximity binding assay -- Calcium mobilization -- Actin polymerization -- Reative oxygen release -- Histamine release -- Measurement of phosphoinositide 3-kinase activity -- Glycosaminogylcan binding assays -- CFU-A assay for measurement of the antiproliferative effects of chemokines on murine early hemopoietic progenitors -- Downmodulation and recycling of chemokine receptors -- Analysis of chemokine receptor endocytosis and recycling -- Chemokine inhibition of HIV infection -- The production of chemokine specific monoclonal antibodies: chemokine RANTES -- Monoclonal antibiotics to chemokine receptors -- Targeted expression of chemokines in vivo -- Chemokine knockout mice -- Chemokine receptor knockout mice -- Measurement of eosinophil accumulatin in vivo
