Antigen processing and presentation protocols

Purification of 20S proteasomes -- Use of proteasome inhibitors to examine processing of antigens for major histocompatibility complex class I presentation -- Tracing the route taken by peptides and major histocompatibility complex class I molecules in presentation of exogenous antigens -- Functional analysis of antigen processing and major histocompatibility complex class II-restricted presentation -- Quantitation of major histocompatibility complex antigen endocytosis using capture ELISA -- In vitro generation of functional human and murine dendritic cells -- Genetic engineering of dendritic cells using retrovirus-based gene transfer techniques -- Use of vaccinia virus expression vectors to investigate antigen processing and presentation -- Sindbis virus-based vectors for the study of class I antigen presentation in vitro and in vivo -- Precision genotyping of human leukocyte antigen-A, -B, and -C loci via direct DNA sequencing -- Photolabeling the transporter associated with antigen processing -- Application of one-dimensional isoelectric focusing to separate different major histocompatibility complex class I alleles and determine their allelic interactions with transporter associated with antigen processing (TAP) -- Analysis of major histocompatibility complex class I interactions with endoplasmic reticulum proteins -- Methods of purification of heat shock protein-peptide complexes for use as vaccines against cancers and infectious diseases -- Random peptide libraries: a tool for analyzing peptide specificity of major histocompatibility complex class I molecules -- Neural network method for predicting peptides that bind major histocompatibility complex molecules -- Analysis of T-cell response using altered peptide ligands -- Assays of thymic selection: fetal thymus organ culture and in vitro thymocyte dulling assay -- Protocol for diversion of a CD4+ response to the T-Helper 2 cell pathway -- Quantitating presentation of MHC class I-restricted antigens -- Identification of CD8+ T-cell-stimulating antigen genes in cDNA libraries -- Generation of antigen-specific, lacZ-inducible T-cell hybrids