Genomics protocols

Machine generated contents note: 1 Construction of Microsatellite-Based, High-Resolution Genetic Maps in the Mouse -- Paul A. Lyons -- 2 Genetic Analysis of Complex Traits -- Stephen P. Bryant and Mathias N. Chiano -- 3 Sequence-Based Detection of Single Nucleotide Polymorphisms -- Deborah A. Nickerson, Natali Kolker, Scott L. Taylor, -- and Mark J. Rieder -- 4 Genomic Mismatch Scanning for the Mapping of Genetic Traits -- Farideh Mirzayans and Michael A. Walter -- 5 Detection of Chromosomal Abnormalities by Comparative Genomic Hybridization -- Mario A.J.A. Hermsen, Marjan M. Weiss, Gerrit A. Meijer, -- and Jan P.A. Baak -- 6 Construction of a Bacterial Artificial Chromosome Library -- Sangdun Choi and Ung-Jin Kim -- 7 Contiguation of Bacterial Clones -- Sean J. Humphray, Susan J. Knaggs, -- and loannis Ragoussis -- 8 Mapping of Genomic Clones by Fluorescence In Situ Hybridization -- Margaret A. Leversha -- 9 Map Integration: From a Genetic Map to a Physical Gene Map and Ultimately to the Sequence Map -- Panagiotis Deloukas -- 10 Construction of Full-Lengin-tnriched cDNA Libraries: The Oligo-Capping Method -- Yutaka Suzuki and Sumio Sugano -- 11 Construction of Transcript Maps by Somatic Cell/Radiation Hybrid Mapping: The Human Gene Map -- Panagiotis Deloukas -- 12 Preparation and Screening of High-Density cDNA Arrays with Genomic Clones -- Gunther Zehetner, Maria Pack, and Katja Schafer -- 13 Direct Selection of cDNAs by Genomic Clones -- Daniela Toniolo -- 14 Exon Trapping: Application of a Large-Insert Multiple-Exon-Trapping System -- Martin C. Wapenaar and Johan T. Den Dunnen -- 15 Sequencing Bacterial Artificial Chromosomes -- David E. Harris and Lee Murphy -- 16 Finding Genes in Genomic Nucleotide Sequences by Using Bioinformatics -- Yvonne J.K. Edwards and Simon M. Brocklehurst -- 17 Gene Identification Using the Pufferfish, Fugu rubripes, by Sequence Scanning -- Greg Elgar -- 18 Isolation of Differentially Expressed Genes Through Subtractive Suppression Hybridization -- Oliver Dorian von Stein -- 19 Isolation of Differentially Expressed Genes by Representational Difference Analysis -- Christine Wallrapp and Thomas M. Gress -- 20 Expression Profiling and the Isolation of Differentially Expressed Genes by Indexing-Based Differential Display -- Michael P. Starkey -- 21 Expression Profiling by Systematic High-Throughput In Situ Hybridization to Whole-Mount Embryos -- Nicolas Pollet and Christof Niehrs -- 22 Expression Monitoring Using cDNA Microarrays: A General Protocol -- Xing Jian Lou, Mark Schena, Frank T. Horrigan, -- Richard M. Lawn, and Ronald W. Davis -- 23 Prediction of Protein Structure and Function by Using Bioinformatics -- Yvonne J.K. Edwards and Amanda Cottage -- 24 Identification of Novel Genes by Gene Trap Mutagenesis -- Anne K. Voss and Tim Thomas -- 25 Determination of Gene Function by Homologous Recombination Using Embryonic Stem Cells and Knockout Mice -- Ahmed Mansouri -- 26 Gehomic Analysis Utilizing the Yeast Two-Hybrid System -- llya G. Serebriiskii, Garabet G. Toby, Russell L. Finley, Jr., -- and Erica A. Golemis -- 27 Methods for Adeno-Associated Virus-Mediated Gene Transfer into Muscle -- Terry J. Amiss and Richard Jude Samulski -- 28 Retroviral-Mediated Gene Transduction -- Donald S. Anson -- 29 Gene Therapy Approaches to Sensitization of Human Prostate Carcinoma to Cisplatin by Adenoviral Expression of p53 and by Antisense Jun Kinase Oligonucleotide Methods -- Ruth Gjerset, All Haghighi, Svetlana Lebedeva, and Dan Mercola -- 30 Ribozyme Gene Therapy -- Leonidas A. Phylactou