Antibody phage display

Preface .......................................................................................................... v Contributors .................................................................................................. xi 1 Overview of Antibody Phage-Display Technology and Its Applications Hennie R. Hoogenboom ..................................................................... 1 2 Standard Protocols for the Construction of Fab Libraries Michelle A. Clark ............................................................................... 39 3 Standard Protocols for the Construction of scFv Libraries Simon Lennard .................................................................................. 59 4 Broadening the Impact of Antibody Phage Display Technology: Amplification of Immunoglobulin Sequences from Species Other than Humans or Mice Philippa M. O’Brien and Robert Aitken .......................................... 73 5 Construction of Large Naïve Fab Libraries Hans J. W. de Haard ......................................................................... 87 6 Construction of Polyclonal Antibody Libraries Using Phage Display Jacqueline Sharon, Seshi R. Sompuram, Chiou-Ying Yang, Brent R. Williams, and Stefanie Sarantopoulos .....................101 7 Antigen-Driven Stimulation of B-Lymphocytes In Vitro Zhiwei Hu .........................................................................................113 8 The Recovery of Immunoglobulin Sequences from Single Human B Cells by Clonal Expansion Ruud M. T. de Wildt and René M. A. Hoet ....................................121 9 Panning of Antibody Phage-Display Libraries: Standard Protocols David W. J. Coomber ......................................................................133 10 Selection of Antibodies Against Biotinylated Antigens Patrick Chames, Hennie R. Hoogenboom, and Paula Henderikx .................................................................. 147 11 Isolation of Anti-Hapten Specific Antibody Fragments from Combinatorial Libraries Keith A. Charlton and Andrew J. Porter ......................................159 12 Blocking Immunodominant Epitopes by Competitive Deselection Roberto Burioni ...............................................................................173 13 Rescue of a Broader Range of Antibody Specificities Using an Epitope-Masking Strategy Henrik J. Ditzel .................................................................................. 179 14 Screening of Phage-Expressed Antibody Libraries by Capture Lift Jeffry D. Watkins ............................................................................... 187 15 Antibody-Guided Selection Using Capture-Sandwich ELISA Kunihiko Itoh and Toshio Suzuki ................................................... 195 16 Proximity-Guided (ProxiMol) Antibody Selection Jane K. Osbourn ............................................................................... 201 17 Isolation of Human Monoclonal Antibodies Using Guided Selection with Mouse Monoclonal Antibodies Mariangela Figini and Silvana Canevari ........................................ 207 18 Selecting Antibodies to Cell-Surface Antigens Using Magnetic Sorting Techniques Don L. Siegel ..................................................................................... 219 19 Isolation of Human Tumor-Associated Cell Surface Antigen-Binding scFvs Elvyra J. Noronha, Xinhui Wang, and Soldano Ferrone .............. 227 20 Subtractive Isolation of Single-Chain Antibodies Using Tissue Fragments Katarina Radosevic and Willem van Ewijk .................................... 235 21 Selection of Antibodies Based on Antibody Kinetic Binding Properties Ann-Christin Malmborg, Nina Nilsson, and Mats Ohlin .............. 245 22 Selection of Functional Antibodies on the Basis of Valency Manuela Zaccolo ............................................................................... 255 23 Two-Step Strategy for Alteration of Immunoglobulin Specificity by In Vitro Mutagenesis Yoshitaka Iba, Chie Miyazaki, and Yoshikazu Kurosawa ............ 259 24 Targeting Random Mutations to Hotspots in Antibody Variable Domains for Affinity Improvement Partha S. Chowdhury ....................................................................... 269 25 Error-Prone Polymerase Chain Reaction for Modification of scFvs Pierre Martineau ................................................................................ 287 26 Use of Escherichia coli Mutator Cells to Mature Antibodies Robert A. Irving, Gregory Coia, Anna Raicevic, and Peter J. Hudson .................................................................... 295 27 Chain Shuffling to Modify Properties of Recombinant Immunoglobulins Johan Lantto, Pernilla Jirholt, Yvelise Barrios, and Mats Ohlin .............................................................................. 303 v viii Contents ´ Contents ix 28 Generation of Bispecific and Tandem Diabodies Sergey M. Kipriyanov ....................................................................... 317 29 High-Level Periplasmic Expression and Purification of scFvs Sergey M. Kipriyanov ....................................................................... 333 30 Periplasmic Expression and Purification of Recombinant Fabs Robert L. Raffaï ................................................................................. 343 31 Expression of Antibody Fragments in Pichia pastoris Philipp Holliger ................................................................................. 349 32 Expression of VHH Antibody Fragments in Saccharomyces cerevisiae J. Marcel van der Vaart .................................................................... 359 33 Intrabodies: Targeting scFv Expression to Eukaryotic Intracellular Compartments Pascale A. Cohen .............................................................................. 367 34 Expression of scFvs and scFv Fusion Proteins in Eukaryotic Cells Michelle de Graaf, Ida H. van der Meulen-Muileman, Herbert M. Pinedo, and Hidde J. Haisma .................................. 379 35 Expression of Antibody Fab Fragments and Whole Immunoglobulin in Mammalian Cells Pietro P. Sanna ................................................................................. 389 Index ............................................................................................................ 397