Mitochondrial DNA : methods and protocols

pt. 1. Methods for the analysis of mtDNA -- Animal models for mitochondrial disease / Douglas C. Wallace -- Identification of mutations in mtDNA from patients suffering mitochondrial diseases / Eric A. Schon -- Screening for aging-dependent point mutations in mtDNA / Yuichi Michikawa -- Scanning low-frequency point mutants in the mitochondrial genome using constant denaturant capillary electrophoresis / Weiming Zheng -- Identification of mtDNA mutations in human cancer / Shuji Nomoto -- In situ PCR of the common human mt DNA deletion: is it related to apoptosis? / Steven J Zullo -- Reproducible quantitative PCR of mitochondrial and nuclear DNA copy number using the LightCycler / Alice Wong -- Yeast nuclear genes for mtDNA maintenance / Francoise Foury -- Measuring mtDNA mutation rates in Saccharomyces cerevisiae using the mtArg8 assay / Micheline K. Strand -- Measuring oxidative mtDNA damage and repair using quantitative PCR / Janine H. Santos -- Migration of mtDNA into the nucleus / Mary K. Thorsness -- 2D gel electrophoresis of mtDNA / Heather E. Lorimer -- pt. 2. Methods for the analysis of proteins involved in mtDNA transactions -- Purifications of mitochondria for enzymes involved in nucleic acid transactions / Daniel F. Bogenhagen -- Purification of mitochondrial uracil-DNA glycosylase using Ugi-sepharose affinity chromatography / Samuel E. Bennett -- Characterization of specialized mtDNA glycosylases / Simon G. Nyaga -- Purification, separation, and identification of the human mtDNA polymerase with and without its accessory subunit / Matthew J. Longley -- Assay of mtDNA polymerase y from human tissues / Robert K. Naviaux -- Purification strategies for Drosophila mtDNA replication proteins in native and recombinant form: DNA polymerase y / Carol L. Farr -- Purification strategies for Drosophila mtDNA replication proteins in native and recombinant form: mitochondrial single-stranded DNA-binding protein / Carol L. Farr -- Expression, purification, and in vitro assays of mitochondrial single-stranded DNA-binding protein / Kang Li -- Recombinant yeast mtDNA helicases: purification and functional assays / Silja Kuusk -- Isolation of DNA topoisomerases I and II activities from mitochondria of mammalian heart / Robert L. Low -- Endonuclease G isolation and assays / Robert L. Low -- Targeting DNA repair proteins to mitochondria / Allison W. Dobson -- In organello footprinting of mtDNA / Steven C. Ghivizzani -- Crosslinking of proteins to mtDNA / Brett A. Kaufman -- Breeding and genotyping ot Tfam conditional knockout mice / Mats Ekstrand