Techniques in aquatic toxicology, volume 2

Contents Section I: Techniques for assessment of toxicity in whole organisms Chapter 1 Integrative measures of toxicant exposure in zebra fish (Danio rerio) at different levels of biological organization ......................................................... 3 Roel Smolders, Wim De Coen, and Ronny Blust Chapter 2 Use of disease challenge assay to assess immunotoxicity of xenobiotics in fish .................................................................................................... 19 Mary R. Arkoosh, Deborah Boylen, Coral L. Stafford, Lyndal L. Johnson, and Tracy K. Collier Chapter 3 Enhanced frog embryo teratogenesis assay: Xenopus model using Xenopus tropicalis ....................................................................................................... 39 Douglas J. Fort and Robert L. Rogers Chapter 4 A short-term mummichog (Fundulus heteroclitus) bioassay to assess endocrine responses to hormone-active compounds and mixtures ............................................................................................................. 55 Deborah L. MacLatchy, Karen L. Gormley, Rebecca E.M. Ibey, Rainie L. Sharpe, Kevin S. Shaughnessy, Simon C. Courtenay, Monique G. Dube´, and Glen J. Van Der Kraak Chapter 5 Conducting dose–response feeding studies with salmonids: Growth as an endpoint .......................................................................................................... 93 James P. Meador, Frank C. Sommers, Leslie Kubin, and Robert J. Wolotira Chapter 6 Field experiments with caged bivalves to assess chronic exposure and toxicity.............................................................................................................. 117 Michael H. Salazar and Sandra M. Salazar Chapter 7 Application of computer microscopy for histopathology in isopod toxicity studies........................................................................................... 137 Damjana Drobne and Samo Drobne Chapter 8 Sperm cell and embryo toxicity tests using the sea urchin Paracentrotus lividus (LmK) ................................................................................... 147 Annamaria Volpi Ghirardini, Alessandra Arizzi Novelli, Chiara Losso, and Pier Francesco Ghetti Chapter 9 Assessment of metal toxicity to sulfate-reducing bacteria through metal concentration methods................................................................ 169 Vivek P. Utgikar and Henry H. Tabak Section II: Techniques for measurement of cellular and subcellular toxicity Chapter 10 Cellular diagnostics and its application to aquatic and marine toxicology................................................................................................. 181 Craig A. Downs Chapter 11 A non-destructive technique to measure cytochrome P4501A enzyme activity in living embryos of the estuarine fish Fundulus heteroclitus ............................................................................................. 209 Diane Nacci, Laura Coiro, Deena M. Wassenberg, and Richard T. Di Giulio Chapter 12 Determination of lipid classes and lipid content in tissues of aquatic organisms using a thin layer chromatography/flame ionization detection (TLC/FID) microlipid method ...................................... 227 Gina M. Ylitalo, Gladys K. Yanagida, Lawrence Hufnagle, Jr., and Margaret M. Krahn Chapter 13 Larval molting hormone synthesis and imaginal disc development in the midge Chironomus riparius as tools for assessing the endocrine modulating potential of chemicals in aquatic insects .................................... 239 Torsten Hahn Chapter 14 The electro-olfactogram: An in vivo measure of peripheral olfactory function and sublethal neurotoxicity in fish................................... 257 David H. Baldwin and Nathaniel L. Scholz Chapter 15 Enzyme-linked immunosorbent assay for screening estrogen receptor binding activity..................................................................... 277 Tomoko Koda, Yoshihiro Soya, and Masatoshi Morita Chapter 16 Lysosomal destabilization assays for estuarine organisms........................... 287 A.H. Ringwood, D.E. Conners, J. Hoguet, and L.A. Ringwood Chapter 17 IMCOMP-P: An assay for coral immuno-competence .................................. 301 Craig A. Downs, Aaron G. Downs, Robert B. Jonas, Kay Briggs, Thomas Capo, and Cheryl M. Woodley Chapter 18 Monitoring gene expression in Rana catesbeiana tadpoles using a tail fin biopsy technique and its application to the detection of environmental endocrine disruptor effects in wildlife species ............................................... 315 Nik Veldhoen and Caren C. Helbing xviii Contents Section III: Techniques for identification and assessment of contaminants in aquatic ecosystems Chapter 19 Coral reproduction and recruitment as tools for studying the ecotoxicology of coral reef ecosystems............................................................. 331 Robert H. Richmond Chapter 20 Using the stickleback to monitor androgens and anti-androgens in the aquatic environment................................................................................. 339 Ioanna Katsiadaki Chapter 21 Simple methods for estimating exposure concentrations of pesticide resulting from non-point source applications in agricultural drainage networks................................................................................................ 357 Wenlin Chen Chapter 22 Design and analysis of toxicity tests for the development and validation of biotic ligand models for predicting metal bioavailability and toxicity ................................................................................. 385 Karel A.C. DeSchamphelaere, Colin R. Janssen, and Dagobert G. Heijerick Chapter 23 Rapid toxicity fingerprinting of polluted waters using lux-marked bacteria ............................................................................................. 409 Nigel L. Turner Chapter 24 Aquatic in situ bioassays to detect agricultural non-point source pesticide pollution: A link between laboratory and field................. 427 Ralf Schulz Chapter 25 Improvements to high-performance liquid chromatography/ photodiode array detection (HPLC/PDA) method that measures dioxin-like polychlorinated biphenyls and other selected organochlorines in marine biota........................................................................ 449 Gina M. Ylitalo, Jon Buzitis, Daryle Boyd, David P. Herman, Karen L. Tilbury, and Margaret M. Krahn Chapter 26 Estrogenic activity measurement in wastewater using in vitro and in vivo methods ............................................................................... 465 Yelena Sapozhnikova, Daniel Schlenk, Anne McElroy, Shane Snyder Chapter 27 A toxicity assessment approach for evaluation of in-situ bioremediation of PAH contaminated sediments .......................................... 479 Henry H. Tabak, James M. Lazorchak, Jim Ferretti, and Mark E. Smith Chapter 28 Application of solid-phase microextraction fibers as biomimetic sampling devices in ecotoxicology............................................... 511 Roman Lanno, and Thomas W. La Point, Jason M. Conder, Jason B. Wells Contents xix Chapter 29 Passive dosimeters for measurement of ultraviolet radiation in aquatic environments...................................................................................... 525 C.S. Sinclair, R.H. Richmond, E.T. Knobbe, C. Basslear, and G.K. Ostrander Section IV: Techniques for aquatic toxicologists Chapter 30 Spectral models for assessing exposure of fish to contaminants ................. 537 Donald C. Malins, Virginia M. Green, Naomi K. Gilman, Katie M. Anderson, and John J. Stegeman Chapter 31 Design and use of a highly responsive and rigidly controllable hypoxia exposure system ............................................................. 547 D.W. Lehmann, J.F. Levine, and J.M. Law Chapter 32 Fish models in behavioral toxicology: Automated techniques, updates and perspectives ................................................................................... 559 Andrew S. Kane, James D. Salierno, and Sandra K. Brewer Chapter 33 Measuring metals and metalloids in water, sediment, and biological tissues................................................................................................... 591 Michael C. Newman and Yuan Zhao Chapter 34 Estimation of inorganic species aquatic toxicity............................................. 617 James P. Hickey Chapter 35 Determining aromatic hydrocarbons and chlorinated hydrocarbons in sediments and tissues using accelerated solvent extraction and gas chromatography/mass spectrometry ............................................................... 631 C.A. Sloan, D.W. Brown, R.W. Pearce, R.H. Boyer, J.L. Bolton, D.G. Burrows, D.P. Herman, and M.M. Krahn Chapter 36 Histological preparation of invertebrates for evaluating contaminant effects...................................................................................................................... 653 Esther C. Peters, Kathy L. Price, and Doranne J. Borsay Horowitz Chapter 37 Isolation of genes in aquatic animals using reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends ............................................................................................................ 687 Jeanette M. Rotchell Chapter 38 Analysis of mutations in l transgenic medaka using the cII mutation assay ....................................................................................................................... 705 Richard N. Winn and Michelle B. Norris Chapter 39 Improved methods of conducting microalgal bioassays using flow cytometry...................................................................................................... 735 Natasha M. Franklin, Jennifer L. Stauber, and Merrin S. Adams Index ........................................................................................................................................... 757