Protein kinase C protocols

The ins and outs of protein kinase c / A.C. Newton - - Early studies of protein kinase c: A historical perspective / Y. Nishizuka and U. Kikkawa - - Expression and purification of protein kinase c from insect cells / H. Mukai and Y. Ono - - Expression and purification of protein kinase cdelta from bacteria / M. Gschwendt and L. Stempka - - Complexities in protein kinase c activity assays: An introduction / J.J. Sando - - Enzyme assays for protein kinase c activity / J.J. Sando and J.K. Beals - - Subcellular translocation of protein kinase c / J.J. Sando, J.K. Beals and I.M. Hussaini - - Measuring the interaction of protein kinase c with membranes: An introduction / A.C. Newton - - Fluorescence imaging of protein kinase c translocation in living cells / N. Saito - - Measuring the binding of protein kinase c to sucrose-loaded vesicles / J. Giorgione and A.C. Newton - - Use of stopped-flow fluorescence spectroscopy to measure rapid membrane binding by protein kinase c / E.A. Nalefski and A.C. Newton - - [3h]phorbol 12,13-dibutyrate binding assay for protein kinase c and related proteins / N.E. Lewin and P.M. Blumberg - - Protein kinase c phosphorylation: An introduction / P.J. Parker - - Pulse-chase analysis of protein kinase c / M. Takahashi and Y. Ono - - Pdk-1 and protein kinase c phosphorylation / A. Toker - - In vitro autophosphorylation of protein kinase c isozymes / A.M. Pepio and W.S. Sossin - - Tyrosine phosphorylation of protein kinase c / T. Yamamoto ... [et al.] - - Methods to study dephosphorylation of protein kinase c in vivo / K. England and M. Rumsby - - Phosphopeptide-specific antibodies to protein kinase c / W.S. Sossin - - Identifying protein kinase c substrates: An introduction / A. Toker - - A chemical genetic approach for the identification of direct substrates of protein kinases / K. Shah and K.M. Shokat - - Studying the optimal peptide substrate motifs of protein kinase c using oriented peptide libraries / M.B. Yaffe - - Structural analysis of protein kinase c: An introduction / J.H. Hurley - - Bacterial expression and purification of c1 and c2 domains of protein kinase c isoforms / W. Cho ... [et al.] - - Crystallization of the protein kinase cdelta c1b domain / G. Zhang and J.H. Hurley - - Methods for detecting binding proteins: An introduction / G. Endemann and D. Mochly-Rosen - - Detection of protein kinase-binding partners by the yeast two-hybrid analysis / B.Y. Chang and C.A. Cartwright - - Glutathione s-transferase pull-down assay / D. Schechtman, D. Mochly-Rosen and D. Ron - - Overlay method for detecting protein-protein interactions / D. Schechtman ... [et al.] - - An overlay assay for detecting protein kinase c-binding proteins and substrates / S. Jaken - - Functional proteomic analysis of the protein kinase c signaling system / J.M. Pass ... [et al.] - - Pharmacological probes for protein kinase c: An introduction / M.G. Kazanietz - - Applications of inhibitors for protein kinase c and their isoforms / G.X. Shen ... [et al.] - - Phorbol esters as probes for the study of protein kinase c function / M.G. Kazanietz and P.S. Lorenzo - - Irreversible inactivation of protein kinase c isozymes by thiol-reactive peptide substrate analogs / C.A. O'Brian ... [et al.] - - Genetic approaches to studying protein kinase c: An introduction / R.O. Messing - - Animal models in the study of protein kinase c isozymes / D.S. Choi and R.O. Messing - - Genetic manipulation of protein kinase c in vivo / A. Toker - - Yeast as a host to screen for modulators and regulatory regions of mammalian protein kinase c isoforms / A.M. Parissenti and H. Riedel - - Protein kinase c in disease: Cancer / A.P. Fields and W.C. Gustafson - - Characterization of the role of protein kinase c isozymes in colon carcinogenesis using transgenic mouse models / A.P. Fields, N.R. Murray and W.C. Gustafson - - Alcohol addiction / M.F. Olive