Bioconjugation protocols : strategies and methods

PART I ANTIBODY AND ENZYME CONJUGATES: Streptavidin-Biotin Crosslinking of Therapeutic Enzymes With Carrier Antibodies: Nanoconjugates for Protection Against Endothelial Oxidative Stress / Vladimir V. Shuvaev, Thomas Dziubla, Rainer Wiewrodt, and Vladimir R. Muzykantov -- Characterization of Endothelial Internalization and Targeting of Antibody-Enzyme Conjugates in Cell Cultures and in Laboratory Animals / Silvia Muro, Vladimir R. Muzykantov, and Juan-Carlos Murciano -- Smart Polymer-Streptavidin Conjugates / Patrick S. Stayton, Zhongli Ding, and Allan S. Hoffman -- Conjugates of Peptides and Proteins to Polyethylene Glycols / Margherita Morpurgo and Francesco M. Veronese -- Chemical Production of Bispecific Antibodies / Robert F. Graziano and Paul Guptill --Preparation of Immunoconjugates Using Antibody Oligosaccharide Moieties / Carl-Wilhelm Vogel -- Synthesis of Hapten-Protein Conjugates Using Microbial Transglutaminase Markus Meusel -- PART II NUCLEIC ACID CONJUGATES: Fluorescent Sample Labeling for DNA Microarray Analyses / Verena Beier, Andrea Bauer, Michael Baum, and Jorg D. Hoheisel -- High-Density Labeling of DNA for Single Molecule Sequencing / Susanne Brakmann -- Sequence-Specific DNA Labeling Using Methyltransferases / Goran Pljevaljtic, Falk Schmidt, Alexander Peschlow, and Elmar Weinhold -- Hapten Labeling of Nucleic Acids for Immuno-Polymerase Chain Reaction Applications / Michael Adler -- Covalent Coupling of DNA Oligonucleotides and Streptavidin / Florian Kukolka, Marina Lovrinovic, Ron Wacker, and Christof M. Niemeyer -- Synthesis of Oligonucleotide-Peptide and Oligonucleotide-Protein Conjugates / David R. Corey -- Synthesis of Peptide Nucleic Acid-Peptide Conjugates / Kunihiro Kaihatsu and David R. Corey -- PART III GLYCOSYL AND LIPID CONJUGATES: Protein Lipidation / lirgen Kuhlmann -- Synthesis of Lipidated Peptides / Ines Heinemann, Martin Volkert, and Herbert Waldmann -- In Vitro Semisynthesis and Applications of C-Terminally Modified Rab Proteins / Thomas Durek, Roger S. Goody, and Kirill Alexandrov -- Generation and Characterization of Ras Lipoproteins Based on Chemical Coupling / Melanie Wagner and iirgen Kuhlmann -- Conjugation of Glycopeptide Thioesters to Expressed Protein Fragments: Semisynthesis of Glycosylated Interleukin-2 / Thomas J. Tolbert and Chi-Huey Wong -- Subtilisin-Catalyzed Glycopeptide Condensation / Thomas J. Tolbert and Chi-Huey Wong -- PART IV BIOFUNCTIONALIZATION OF SURFACES: Peptide Nucleic Acid Microarrays / Anette Jacob, Ole Brandt, Achim Stephan, and forg D. Hoheisel ... 283 22 Synthesis and Characterization of Deoxyribonucleic Acid-Conjugated Gold Nanoparticles / Pompi Hazarika, Tatiana Giorgi, Martina Reibner, Buelent Ceyhan, and Christof M. Niemeyer -- Biofunctionalization of Carbon Nanotubes for Atomic Force Microscopy Imaging / Adam T. Woolley.