Platelets and megakaryocytes. Vol. 1, Functional assays

Functional Assays Preface ............................................................................................................. v Contents of Volume II ................................................................................... xiii Contributors ................................................................................................... xv Color Plates ................................................................................................... xix PART I. PLATELET FUNCTIONAL ASSAYS 1 Effects of Anticoagulants Used During Blood Collection on Human Platelet Function Jane A. May and Stanley Heptinstall .................................................... 3 2 Preparation of Washed Platelet Suspensions From Human and Rodent Blood Jean-Pierre Cazenave, Philippe Ohlmann, Dominique Cassel, Anita Eckly, Béatrice Hechler, and Christian Gachet .................... 13 3 Platelet Counting Paul Harrison, Carol Briggs, and Samuel J. Machin ........................... 29 4 Electron Microscopy Methods for Studying Platelet Structure and Function James G. White ................................................................................... 47 5 Platelet Aggregation: Turbidimetric Measurements Gavin E. Jarvis ..................................................................................... 65 6 Platelet Aggregation in Whole Blood: Impedance and Particle Counting Methods Gavin E. Jarvis ..................................................................................... 77 7 Secretion From Dense Granules: Luminescence Method for Adenine Nucleotides M. Fred Heath ..................................................................................... 89 8 Platelet Dense-Granule Secretion: The [3H]-5-HT Secretion Assay David Crosby and Alastair W. Poole .................................................. 95 9 Analysis of Releasable Nucleotides of Platelets Joachim Jankowski, Lars Henning, and Hartmut Schlüter ................. 97 10 Studies of Secretion Using Permeabilized Platelets Tara W. Rutledge and Sidney W. Whiteheart .................................. 109 11 Measurement of Platelet Arachidonic Acid Metabolism Richard W. Farndale, Philip G. Hargreaves, Joanna L. Dietrich, and Rosemary J. Keogh ................................ 121 ix x Contents 12 Measurement of the Platelet Procoagulant Response Johan W. M. Heemskerk, Paul Comfurius, Marion A. H. Feijge, and Edouard M. Bevers ................................................................. 135 13 Platelet Adhesion Assays Performed Under Static Conditions Joanne M. Stevens ............................................................................. 145 14 Platelet Adhesion Assays Under Flow Using Matrix Protein-Coupled Adhesion Columns Renata Polanowska-Grabowska and Adrian R. L. Gear ................... 153 15 Techniques to Examine Platelet Adhesive Interactions Under Flow Suhasini Kulkarni, Warwick S. Nesbitt, Sacha M. Dopheide, Sascha C. Hughan, Ian S. Harper, and Shaun P. Jackson ............. 165 16 In Vivo Models of Platelet Function and Thrombosis: Study of Real-Time Thrombus Formation Shahrokh Falati, Peter L. Gross, Glenn Merrill-Skoloff, Derek Sim, Robert Flaumenhaft, Alessandro Celi, Barbara C. Furie, and Bruce Furie ................................................ 187 17 Adhesion Between Platelets and Leukocytes or Endothelial Cells Gerard B. Nash ................................................................................. 199 18 In Vitro Measurement of High-Shear Platelet Adhesion and Aggregation by the PFA-100® Paul Harrison .................................................................................... 215 19 Flow-Cytometric Analysis of Platelet-Membrane Glycoprotein Expression and Platelet Activation Alison H. Goodall and Jackie Appleby ............................................. 225 20 Flow-Cytometric Analysis of Mouse Platelet Function Bernhard Nieswandt, Valerie Schulte, and Wolfgang Bergmeier .... 255 21 Platelet Microparticles Shosaku Nomura and Shirou Fukuhara ............................................ 269 PART II. MEGAKARYOCYTE FUNCTIONAL ASSAYS 22 Isolation of Primary Megakaryocytes and Studies of Proplatelet Formation Robert M. Leven ................................................................................ 281 23 Isolation and Culture of Megakaryocyte Precursors Najet Debili, Fawzia Louache, and William Vainchenker ............... 293 24 Assays of Megakaryocyte Development: Surface Antigen Expression, Ploidy, and Size Anthony Mathur, Ying Hong, Guosu Wang, and Jorge D. Erusalimsky .............................................................. 309 25 Assays of Megakaryocyte Development: Cytoplasm, Storage Granules, and Demarcation Membranes Arnaud Drouin, Gulie Alimardani, and Elisabeth M. Cramer .......... 323 26 Thrombopoietin Bioassay Warren S. Alexander and Craig Hyland ........................................... 347 27 Culture of Megakaryocytic Cell Lines: Uses and Limitations Norio Komatsu .................................................................................. 361 Index ............................................................................................................ 375