Platelets and megakaryocytes

v. 1. Functional assays -- v. 2. Perspectives and techniques. v. 1. Effects of anticoagulants used during blood collection on human platelet function / J.A. May and S. Heptinstall -- Preparation of washed platelet suspensions from human and rodent blood / J.P. Cazenave ... [et al.] -- Platelet counting / P. Harrison, C. Briggs and S.J. Machin -- Electron microscopy methods for studying platelet structure and function / J.G. White -- Platelet aggregation: Turbidimetric measurements / G.E. Jarvis -- Platelet aggregation in whole blood: Impedance and particle counting methods / G.E. Jarvis -- Secretion from dense granules: Luminescence method for adenine nucleotides / M.F. Heath -- Platelet dense-granule secretion: The [3h]-5-ht secretion assay / D. Crosby and A.W. Poole -- Analysis of the releasable nucleotides of platelets / J. Jankowski, L. Henning and H. Schluter -- Studies of secretion using permeabilized platelets / T.W. Rutledge and S.W. Whiteheart -- Measurement of platelet arachidonic acid metabolism / R.W. Farndale ... [et al.] -- Measurement of the platelet procoagulant response / J.W. Heemskerk ... [et al.] -- Platelet adhesion assays performed under static conditions / J.M. Stevens -- Platelet adhesion assays under flow using matrix protein-coupled adhesion columns / R. Polanowska-Grabowska and A.R. Gear -- Techniques to examine platelet adhesive interactions under flow / S. Kulkarni ... [et al.] -- In vivo models of platelet function and thrombosis: Study of real-time thrombus formation / S. Falati ... [et al.] -- Adhesion between platelets and leukocytes or endothelial cells / G.B. Nash -- In vitro measurement of high-shear platelet adhesion and aggregation by the pfa-100 / P. Harrison -- Flow-cytometric analysis of platelet-membrane glycoprotein expression and platelet activation / A.H. Goodall and J. Appleby -- Flow-cytometric analysis of mouse platelet function / B. Nieswandt, V. Schulte and W. Bergmeier -- Platelet microparticles / S. Nomura and S. Fukuhara -- Isolation of primary megakaryocytes and studies of proplatelet formation / R.M. Leven -- Isolation and culture of megakaryocyte precursors / N. Debili, F. Louache and W. Vainchenker -- Assays of megakaryocyte development: Surface antigen expression, ploidy, and size / A. Mathur ... [et al.] -- Assays of megakaryocyte development: Cytoplasm, storage granules, and demarcation membranes / A. Drouin, G. Alimardani and E.M. Cramer -- Thrombopoietin bioassay / W.S. Alexander and C. Hyland -- Culture of megakaryocytic cell lines: Uses and limitations / N. Komatsu. v. 2. Signaling receptors on platelets and megakaryocytes / D. Woulfe ... [et al.] -- Manipulation of gene expression in megakaryocytes / N.R. Emambokus, G.J. Murphy and J. Frampton -- In vitro changes of platelet parameters: Lessons from blood banking / J. Rivera, M.L. Lozano and V. Vicente -- Pharmacological approaches to studying platelet function: An overview / S.M. Hourani -- Inhibitors of cellular signaling targets: Designs and limitations / C.J. Vlahos and L.F. Stancato -- Ligand-binding assays for collagen / S.M. Jung and M. Moroi -- Use of radiolabeled 2-methylthio-adp to study p2y receptors on platelets and cell lines / P. Savi and J.M. Herbert -- Ligand-binding assays: Fibrinogen / D. Cox -- Techniques for analysis of proteins by sds-polyacrylamide gel electrophoresis and western blotting / J.M. Gibbins -- Study of tyrosine kinases and protein tyrosine phosphorylation / J.M. Gibbins -- Protein tyrosine phosphatases / M.L. Jones and A.W. Poole -- The study of serine-threonine kinases / G. Gorter and J. Willem Akkerman -- Phosphoinositides: Lipid kinases and phosphatases / B. Payrastre -- Isolation and analysis of platelet lipid rafts / C.N. Shrimpton ... [et al.] -- Measurement and manipulation of [ca2+]i in suspensions of platelets and cell cultures / P. Ohlmann ... [et al.] -- Measurement and manipulation of intracellular ca2+ in single platelets and megakaryocytes / M.J. Mason and M.P. Mahaut-Smith -- Patch-clamp recordings of electrophysiological events in the platelet and megakaryocyte / M.P. Mahaut-Smith -- Evaluation of nitrotyrosine-containing proteins in blood platelets / K.M. Naseem, R. Riba and M. Troxler -- Nitric oxide signaling in platelets / S.Y. Low and K.R. Bruckdorfer -- Snake venom toxins affecting platelet function / R.K. Andrews, E.E. Gardiner and M.C. Berndt -- Peptide synthesis in the study of collagen-platelet interactions / C.G. Knight, C.M. Onley and R.W. Farndale -- Platelet permeabilization / R. Flaumenhaft -- Using retroviruses to express genes in primary megakaryocyte lineage cells / M. Gaur ... [et al.] -- Use of antisense oligonucleotide technology to investigate signaling pathways in megakaryocytes / H. Avraham, S. Avraham and R. Zagozdzon -- Gfp fusion proteins to study signaling in live cells / S.A. Walker, G.E. Cozier and P.J. Cullen -- Two-dimensional polyacrylamide gel electrophoresis for platelet proteomics / K. Marcus and H.E. Meyer -- Preparation of mrna and cdna libraries from platelets and megakaryocytes / B.Z. Paul, J. Jin and S.P. Kunapuli -- Platelet receptor structures and polymorphisms / T.J. Kunicki, S. Head and D.R. Salomon -- Gene array technology and the study of platelets and megakaryocytes / L.T. Lam and E.H. Bresnick.