Protein-protein interactions : methods and applications

Structural bases of protein-protein interactions / Robert C. Liddington -- Quantitative analysis of protein-protein interactions / Keith D. Wilkinson -- Characterization of protein-protein interactions by isothermal titration calorimetry / Adrian Velazquez-Campoy, Stephanie A. Leavitt, and Ernesto Freire -- Circular dichroism analysis for protein-protein interactions / Norma J. Greenfield -- Protein-protein interaction analysis by nuclear magnetic resonance spectroscopy / Guanghua Gao, Jason G. Williams, and Sharon L. Campbell -- Measuring rhodopsin-G-protein interactions by surface plasmon resonance / John Northup -- Using light scattering to determine the stoichiometry of protein complexes / Jeremy Mogridge -- Sedimentation, equilibrium studies / Ian A. Taylor, John F. Eccleston, and Katrin Rittinger -- Analysis of protein-protein interactions by simulation of small-zone gel filtration chromatography / Rosemarie Wilton, Elizabeth A. Myatt, and Fred J. Stevens -- Fluorescence gel retardation assay to detect protein-protein interactions / Sang-Hyun Park and Ronald T. Raines -- Fluorescence polarization assay to quantify protein-protein interactions / Sang-Hyun Park and Ronald T. Raines -- Studying protein-protein interactions via blot overlay of far western blot / Randy A. Hall -- Glutathione-S-transference-fusion based assays for studying protein-protein interactions / Haris G. Vikis and Kun-Liang Guan. Affinity capillary electrophoresis analyses of protein-protein interactions in target-directed drug discovery / William E. Pierceall, Lixin Zhang, and Dallas E. Hughes -- Mapping protein-ligand interactions by hydroxyl-radical protein footprinting / Nick Loizos -- Use of phage display and polyvalency to design inhibitors of protein-protein interactions / Micheal Mourez and R. John Collier -- A bacterial two-hybrid system based on transcriptional activation / Simon L. Dove and Ann Hochschild -- Using the yeast two-hybrid system to indentify interacting proteins / John Miller and Igor Stagljar -- Analysis of protein-protein interactions utilizing dual bait yeast two-hybrid system / Ilya G. Serebriiskii and Elena Kotova -- The split-ubiquitin membrane-based yeast two-hybrid system / Safia Thaminy, John Miller, and Igor Stagljar -- Reverse two-hybrid techniques in the yeast Saccharomyces cerevisiae / Matthew A. Bennett, Jack F. Shern, and Richard A. Kahn -- Mammalian two-hybrid assay for detecting protein-protein interactions in vovo / Jae Woon Lee and Soo-Kyung Lee -- Co-immunoprecipitation from transfected cells / Shane C. Masters -- Microscopic analysis of fluorescence resonance energy transfer (FRET) / Brian Herman, R. Venkata Krishnan, and Victoria E. Centonze. Monitoring molecular interactions in living cells using flow cytometric analysis of fluorescence resonance energy transfer / Francis Ka-Ming Chan -- Fluorescence correlation spectroscopy: a new tool for quantification of molecular interactions / Keith M. Berland -- Confocal microscopy of intracellular co-localization of proteins / Toshiyuki Miyashita -- Mapping biochemical networks with protein-fragment complementation assays / Ingrid Remy and Stephen W. Michnick -- In vivo protein cross-linking / Fabrice Agou, Fei Ye, and Michel Véron -- Computational prediction of protein-protein interactions / John C. Obenauer and Michael B. Yaffe -- Affinity methods for phosphorylation-dependent interactions / Greg Moorhead and Carol MacKintosh -- Two-dimensional gel electrophoresis for analysis of protein complexes / Karin Barnouin -- Sample preparation of gel electrophoretically separated protein binding partners for analysis by mass spectrometry / Rainer Cramer, Malcolm Saxton, and Karin Barnouin -- Quantitative protein analysis by solid phase isotope tagging and mass spectrometry / Huilin Zhou, Rosemary Boyle, and Ruedi Aebersold -- Internet resources for studying protein-protein interactions / Shane C. Masters