Diabetes mellitus : methods and protocols

Isolation of islets of Langerhans from rodent pancreas / C.B. Kelly ... [et al.] -- Purification of rat pancreatic B-cells by fluorescence-activated cell sorting / G. Stange, M. Van De Casteele and H. Heimberg -- Assessment of insulin secretion in the mouse / M. Brissova ... [et al.] -- Detection of insulin production by immunohistochemistry / S.C. Campbell and W.M. Macfarlane -- Quantification of the level of insulin gene expression / S.C. Campbell and W.M. Macfarlane -- Chromatin immunoprecipitation using isolated islets of Langerhans / M. Parrizas ... [et al.] -- Adenoviral gene transfer into beta-cell lines / A.L. Mosley and S. Ozcan -- Utilization of NOD mice in the study of type 1 diabetes / D.G. Karounos and S.E. Goes -- Introduction of DNA into 3T3-L1 adipocytes by electroporation / S. Okada, M. Mori and J.E. Pessin -- Analysis of insulin-stimulated glucose uptake in differentiated 3T3-L1 adipocytes / J. Lakshmanan, J.S. Elmendorf and S. Ozcan -- Fractionation analysis of the subcellular distribution of GLUT-4 in 3T3-L1 adipocytes / J.S. Elmendorf -- Visualization and quantitation of integral membrane proteins using a plasma membrane sheet assay / J.B. Knight and A.L. Olson -- Assaying tyrosine phosphorylation of insulin receptor and insulin receptor substrates / E.L. Whiteman and M.J. Birnbaum -- Measuring insulin-stimulated phosphatidyl-inositol 3-kinase activity / L.P. Wang and S.A. Summers -- Assaying AKT/protein kinase B activity / K.L. Hoehn and S.A. Summers -- Measurements of cellular phosphoinositide levels in 3T3-L1 adipocytes / D.B. DeWald -- Measurement of glycogen synthesis and glycogen synthase activity in 3T3-L1 adipocytes / M.J. Brady -- Measurement of contraction-stimulated GLUT-4 translocation in isolated skeletal muscle / J.T. Brozinick, Jr. -- Single-embryo measurement of basal- and insulin-stimulated glucose uptake / M.M. Chi and K.H. Moley -- Immunohistologic staining of muscle and embryos to detect insulin-stimulated translocation of glucose transporters / M.O. Carayannopoulos and K.H. Moley