MRNA processing and metabolism

MRNA processing and metabolism

  • نوع فایل : کتاب
  • زبان : انگلیسی
  • مؤلف : Daniel R Schoenberg
  • ناشر : Humana Press
  • چاپ و سال / کشور: 2004
  • شابک / ISBN : 9781588292254

Description

Using chromatin immunoprecipitation to map cotranscriptional mRNA processing in saccharomyces cerevisiae / Michael-Christopher Keogh and Stephen Buratowski -- Identifying phosphoCTD-associating proteins / Hemali P. Phatnani and Arno L. Greenleaf -- Imaging alternative splicing in living cells / Eric J. Wagner ... [et al.] -- Tobramycin affinity tag purification of spliceosomes / Klaus Hartmuth, Hans-Peter Vornlocher and Reinhard Lührmann -- Using single-strand conformational polymorphism gel electrophoresis to analyze mutually exclusive alternative splicing / Alicia M. Celotto and Brenton R. Graveley -- Methods for the analysis of adenosine-to-inosine editing in RNA / Zuo Zhang and Gordon G. Carmichael -- Assaying nuclear messenger RNA export in human cells / Bryan R. Cullen -- RNA unwinding assay for DExD/H-Box RNA helicases / Stephanie S. Tseng-Rogenski and Tien-Hsien Chang -- Approaches for monitoring nuclear translation / Francisco J. Iborra, Dean A. Jackson, and Peter R. Cook -- Immunopurification and analysis of protein and RNA components of mRNP in mammalian cells / Fabrice Lejeune and Lynne E. Maquat. -- Gene expression analysis of messenger RNP complexes / Luiz O.F. Penalva, Scott A. Tenenbaum, and Jack D. Keene -- Using the -N peptide to tether proteins to RNAs / Julie Baron-Benhamou ... [et al.] -- An efficient system for cap- and poly(A)-dependent translation in vitro / Yuri V. Svitkin and Nahum Sonenberg -- A poly(A) tail-responsive in vitro system for cap- or IRES-driven translation from HeLa cells / Christian Thoma, Antje Ostareck-Lederer and Matthias W. Hentze -- Assessing messenger RNA decapping in cellular extracts / Naomi Bergman ... [et al.] -- Analysis of RNA exonucleolytic activities in cellular extracts / Devi Mukherjee ... [et al.] -- Application of ligation-mediated reverse transcription polymerase chain reaction to the identification of in vivo endonuclease-generated messenger RNA decay intermediates / Mark N. Hanson and Daniel R. Schoenberg -- Biochemical dissection of RNA silencing in plants / Guiliang Tang and Phillip D. Zamore -- RNA interference of mRNA processing factors in drosophila S2 cells / Alicia M. Celotto and Brenton R. Graveley -- RNA interference by short hairpin RNAs expressed in vertebrate cells / Gregory J. Hannon and Douglas S. Conklin
The chromatin immunoprecipitation (ChIP) technique has been used to determine where and under what conditions DNA binding proteins associate with specific DNA sequences. Proteins are crosslinked in vivo with formaldehyde, and chromatin is then isolated and sheared. The protein of interest is then immunoprecipitated and the associated DNA sequences identified via PCR. Although this technique was originally designed to assay DNA binding proteins, it can also be used to monitor mRNA processing factors associated with transcription complexes.
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