MRNA processing and metabolism : methods and protocols

Using chromatin immunoprecipitation to map cotranscriptional mRNA processing in saccharomyces cerevisiae / Michael-Christopher Keogh and Stephen Buratowski -- Identifying phosphoCTD-associating proteins / Hemali P. Phatnani and Arno L. Greenleaf -- Imaging alternative splicing in living cells / Eric J. Wagner ... [et al.] -- Tobramycin affinity tag purification of spliceosomes / Klaus Hartmuth, Hans-Peter Vornlocher and Reinhard Lührmann -- Using single-strand conformational polymorphism gel electrophoresis to analyze mutually exclusive alternative splicing / Alicia M. Celotto and Brenton R. Graveley -- Methods for the analysis of adenosine-to-inosine editing in RNA / Zuo Zhang and Gordon G. Carmichael -- Assaying nuclear messenger RNA export in human cells / Bryan R. Cullen -- RNA unwinding assay for DExD/H-Box RNA helicases / Stephanie S. Tseng-Rogenski and Tien-Hsien Chang -- Approaches for monitoring nuclear translation / Francisco J. Iborra, Dean A. Jackson, and Peter R. Cook -- Immunopurification and analysis of protein and RNA components of mRNP in mammalian cells / Fabrice Lejeune and Lynne E. Maquat. -- Gene expression analysis of messenger RNP complexes / Luiz O.F. Penalva, Scott A. Tenenbaum, and Jack D. Keene -- Using the -N peptide to tether proteins to RNAs / Julie Baron-Benhamou ... [et al.] -- An efficient system for cap- and poly(A)-dependent translation in vitro / Yuri V. Svitkin and Nahum Sonenberg -- A poly(A) tail-responsive in vitro system for cap- or IRES-driven translation from HeLa cells / Christian Thoma, Antje Ostareck-Lederer and Matthias W. Hentze -- Assessing messenger RNA decapping in cellular extracts / Naomi Bergman ... [et al.] -- Analysis of RNA exonucleolytic activities in cellular extracts / Devi Mukherjee ... [et al.] -- Application of ligation-mediated reverse transcription polymerase chain reaction to the identification of in vivo endonuclease-generated messenger RNA decay intermediates / Mark N. Hanson and Daniel R. Schoenberg -- Biochemical dissection of RNA silencing in plants / Guiliang Tang and Phillip D. Zamore -- RNA interference of mRNA processing factors in drosophila S2 cells / Alicia M. Celotto and Brenton R. Graveley -- RNA interference by short hairpin RNAs expressed in vertebrate cells / Gregory J. Hannon and Douglas S.