Vaccinia virus and poxvirology : methods and protocols

Working safely with Vaccinia virus : laboratory technique and the role of Vaccinia vaccination / Stuart N. Isaacs -- Construction and isolation of recombinant Vaccinia virus using genetic markers / Maria M. Lorenzo, Inmaculada Galindo, and Rafael Blasco -- Construction of recombinant Vaccinia virus : cloning into the thymidine kinase locus / Chelsea M. Byrd and Dennis E. Hruby -- Transient and inducible expression of Vaccinia/T7 recombinant viruses / Mohamed R. Mohamed and Edward G. Niles -- Construction of recombinant Vaccinia viruses using leporipoxvirus catalyzed recombination and reactivation of orthopoxvirus DNA / Xiao-Dan Yao and David H. Evans -- Construction of CDNA libraries in Vaccinia virus / Ernest S. Smith, Shuying Shi, and Maurice Zauderer -- Construction and isolation of recombinant MVA / Caroline Staib, Ingo Drexler, and Gerd Sutter -- Growing poxviruses and determining virus titer / Girish J. Kotwal and Melissa-Rose Abrahams -- Rapid preparation of vaccinia virus DNA template for analysis and cloning by PCR / Rachel L. Roper -- Orthopoxvirus diagnostics / Hermann Meyer, Inger K. Damon, and Joseph J. Esposito -- An in vitro transcription system for studying vaccinia virus early genes / Steven S. Broyles and Marcia Kremer -- An in vitro transcription system for studying vaccinia virus late genes / Cynthia F. Wright -- Studying vaccinia virus RNA processing in vitro / Paul D. Gershon -- Methods for analysis of poxvirus DNA replication / Paula Traktman and Kathleen Boyle -- Studying the binding and entry of the intracellular and extracellular enveloped forms of vaccinia virus / Mansun Law and Geoffrey L. Smith -- Pox, dyes, and videotape: Making movies of GFP-labeled vaccinia virus / Brian M. Ward -- Interaction analysis of viral cytokine-binding proteins using surface plasmon resistance / Bruce T. Seet and Grant McFadden -- Monitoring of human immunological responses to vaccinia virus / Richard Harrop, Matthew G. Ryan, Hana Golding, Irina Redchenko, and Miles W. Carroll -- Vaccinia virus as a tool for immunologic studies / Nia Tatsis, Gomathinayagam Sinna bathamby, and Laurence C. Eisenlohr -- Mouse models for studying orthopoxvirus respiratory infections / Jill Schriewer, R. Mark Buller, and Gelita Owens -- Viral glycoprotein-mediated cell fusion assays using Vaccinia virus vectors / Katharine N. Bossart and Christopher C. Broder -- Use of dual recombinant Vaccinia virus vectors to assay viral glycoprotein-mediated fusion with transfection-resistant primary cell targets / Yanjie Yi, Anjali Singh, Joanne Cutilli, and Ronald G. Collman -- Poxvirus bioinformatics / Chris Upton -- Preparation and use of molluscum contagiosum virus from human tissue biopsy specimens / Nadja V. Melquiot and Joachim J. Bugert. Construction and isolation of recombination MVA / Caroline Staib, Ingo Drexler, and Gerd Sutter -- Growing poxviruses and determining virus titer / Girish J. Kotwal and Melissa Abraham -- Rapid preparation of Vaccinia virus DNA template for analysis and cloning by PCR / Rachel L. Roper -- Orthopoxvirus diagnostics / Hermann Meyer, Inger K. Damon, and Joseph J. Esposito -- An in vitro transcription system for studying Vaccinia virus early genes / Steven S. Broyles and Marcia Kremer -- An in vitro transcription system for studying Vaccinia virus late genes / Cynthia F. Wright -- Studying Vaccinia virus RNA processing in vitro / Paul D. Gershon -- Methods for analysis of poxvirus DNA replication / Paula Traktman and Kathleen Boyle -- Viral glycoprotein-mediated cell fusion assays using Vaccinia virus vectors / Katharine N. Bossart and Christopher C. Broder -- Use of dual recombinant Vaccinia virus vectors to assay viral glycoprotein-mediated fusion with transfection-resistant primary cell targets / Yanjie Yi, Anjali Singh, Joanne Cutilli, and Ronald G. Collman -- Poxvirus bioinformatics / Chris Upton -- Preparation and use of molluscum contagiosum virus from human tissue biopsy specimens / Nadja V. Melquiot and Joachim J. Bugert