Photosynthesis research protocols

Fractionation of thylakoid membranes into grana and stroma thylakoids / Juan Cuello and Maria Jose Quiles -- Isolation of photosystem I particles from spinach / Tetsuo Hiyama -- Rapid isolation and purification of photosystem I chlorophyll-binding protein from Chlamydomonas reinhardtii / Velupillai M. Ramesh and Andrew N. Webber -- Isolation of photosystem II-enriched membranes and the oxygen-evolving complex subunit proteins from higher plants / Yasusi Yamamoto, Shinsuke Sakuma, and Jian-Ren Shen -- Isolation of functional photosystem II core particles from the Cyanobacterium synechocystis sp. PCC 6803 / Dmitrii Vavilin -- Isolation of photosystem I reaction center preparation from spinach / Tetsuo Hiyama -- Isolation of photosystem II reaction center complexes from plants -- Michael Seibert, Inmaculada Yruela, and Rafael Picorel -- Isolation of photosystem I reaction center subunit polypeptide from spinach / Tetsuo Hiyama. Purification of the cytochrome b6f complex in oxygenic photosynthesis / Huamin Zhang and William A. Cramer -- Purification of plastocyanin and cytochrome c6 from plants, green algae, and cyanobacteria / Jose A. Navarro, Manuel Hervçs, and Miguel A. De la Rosa -- Preparation of native and recombinant light-harvesting chlorophyll-a/b complex / Wolfgang Rohle and Harald Paulsen -- Isolation and characterization of lamellar aggregates of LHCII and LHCII-lipid macroassemblies with light-inducible structural transitions / Ilian Simidjiev, Zsuzsanna Vçrkonyi, and GyÜzÜ Garab -- Separation, purification and characterization of polypeptide composition of subcomplexes of the main light-harvesting chlorophyll a/b-protein complex of photosystem II (LHCII) / Grzegorz Jackowski -- Isolation of CP43 and CP47 photosystem II proximal antenna complexes for plants / Rafael Picorel, Miguel Alfonso, and Michael Seibert. The determination and quantification of photosynthetic pigments by reverse phase high performance liquid chromatography (HPLC), thin layer chromatography, and spectroscopy / Tessa Pocock, Marianna Krùl, and Norman P.A. Huner -- Isolation and identification of chloroplast lipids / Sato and Mikio Tsuzuki -- DNA adducts with chlorophyll and chlorophyllin as antimutagenic agents: synthesis, stability, and structural features / Heidar-Ali Tajmir-Riahi, Jean-Francois Neault, and Stravoula Diamantoglou -- Incorporation and analysis of LHCII in model systems / Wieslaw I. Gruszecki -- Photosystem II reconstitution into proteoliposomes: structure-function characterization / Mario Fragata -- Extraction of the functional manganese and calcium cofactors from photosystem II / Joel Freeman, Garth Hendry, and Tom Wydrzynski -- Assay of photoinhibition of photosystem II and protease activity / Yasusi Yamamoto, Yoji Nishi, Hitoshi Yamasaki, Suguru Uchida, and Satoshi Ohira. Thermoluminescence: a technique for probing photosystem II / Prafullachandra V. Sane -- Detection of free radicals and reactive oxygen species / Eva Hideg -- Stabilization of photosynthetic materials / Regis Rouillon, Pierre Euzet, and Robert Carpentier -- Determination of phosphoproteins in higher plant thylakoids / Eva-Mari Aro, Anne Rokka, and Alexander V. Vener -- Identifying photoprotection mutants in Arabidopsis thaliana / Jan Bart Rossel, Abby Cuttriss, and Barry J. Pogson -- A simple method for chloroplast transformation in Chlamydomonas reinhardtii / Velupillai M. Ramesh, Scott E. Bingham, and Andrew N. Webber -- Construction of gene knockouts in the Cyanobacterium synechocystis pcc 6803 / Julian J. Eaton-Rye -- Gene inactivation in the cyanobacterium synechococcus sp. PCC 7002 and the green sulfur bacterium Chlorobium tepidum using in vitro-made DNA constructs and natural transformation / Niels-Ulrik Frigaard, Yumiko Sakuragi, and Donald A. Bryant.