RNA interference, editing, and modification : methods and protocols

RNA interference, editing, and modification : methods and protocols

  • نوع فایل : کتاب
  • زبان : انگلیسی
  • مؤلف : Jonatha M Gott
  • ناشر : Humana Press
  • چاپ و سال / کشور: 2004
  • شابک / ISBN : 9781588292421

Description

RNA interference: Historical overview and significance / M.K. Montgomery -- Methods for delivery of double-stranded rna into caenorhabditis elegans / D. Hull and L. Timmons -- Induction and biochemical purification of rna-induced silencing complex from drosophila s2 cells / A.A. Caudy and G.J. Hannon -- Analysis of gene function in trypanosoma brucei using rna interference / A. Djikeng ... [et al.] -- Short hairpin activated gene silencing in mammalian cells / P.J. Paddison ... [et al.] -- Geminivirus vectors for transient gene silencing in plants / N. Muangsan and D. Robertson -- Posttranscriptional gene silencing in plants / S.V. Wesley ... [et al.] -- Identification of micrornas and other tiny noncoding rnas by cdna cloning / V. Ambros and R.C. Lee -- A historical perspective on rna editing: How the peculiar and bizarre became mainstream / D.J. Koslowsky -- Identification of substrates for adenosine deaminases that act on rna / D.P. Morse -- Purification and assay of recombinant adar proteins expressed in the yeast pichia pastoris or in escherichia coli / G.M. Ring, M.A. O'Connell and L.P. Keegan -- Isolation of an mrna-binding protein involved in c-to-u editing / C.A. Gerber, A. Relich and D.M. Driscoll -- In vitro assays for kinetoplastid u insertion-deletion editing and associated activities / K. Stuart ... [et al.] -- Identification and characterization of trypanosome rna-editing complex components / K. Stuart, A.K. Panigrahi and A. Schnaufer -- Chimeric templates and assays used to study physarum cotranscriptional insertional editing in vitro / E.M. Byrne -- Methods for analysis of mitochondrial trna editing in acanthamoeba castellanii / A.J. Lohan and M.W. Gray -- In vitro rna editing systems from higher plant chloroplasts / T. Hirose ... [et al.] -- Studying rna editing in transgenic chloroplasts of higher plants / R. Bock -- Detection and quantification of modified nucleotides in rna using thin-layer chromatography / H. Grosjean, G. Keith and L. Droogmans -- Functional characterization of 2'-o-methylation and pseudouridylation guide rnas / T. Kiss and B.E. Jady -- Experimental rnomics: A global approach to identifying small nuclear rnas and their targets in different model organisms / A. Huttenhofer, J. Cavaille and J.P. Bachellerie.
In the early 1990s, attempts to manipulate gene expression by researchers working in three different fields resulted in unanticipated gene silencing. Rather than ignoring such results, these researchers went on to document and further investigate the nature of such silencing, which was named “co-suppression” in plants, “quelling” in fungi, and “RNA interference” (RNAi) in nematodes. By the late 1990s, it was discovered that silencing could be initiated in this diverse set of organisms by exposing cells to double-stranded RNA (dsRNA), which directed the destruction of mRNAs containing similar sequences. Soon afterward, such dsRNA-mediated silencing was employed as a reverse genetic technique to analyze the functions of specific genes in a broad variety of organisms. Biochemical and genetic studies designed to uncover the components of the RNA silencing machinery identified a common core of proteins that serve to amplify the interfering RNA signal and direct endonucleolytic cleavage of target RNAs. A subset of silencing events may also direct DNA methylation of targeted genes. RNA silencing is thought to have evolved as a defense mechanism to suppress viral replication and transposon mobilization. However, additional functions involving the RNAi machinery have been uncovered, including posttranscriptional regulation of endogenous genes, and maintenance of structure and function of heterochromatin. Whereas many researchers have focused on understanding the natural biological functions of RNA silencing, others are testing its utility in antiviral and cancer therapies and in other biotechnological and biomedical applications
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