Signal transduction protocols

Making protein immunoprecipitates / Elaine A. Elion and Yunmei Wang -- Signal transduction inhibitors in cellular function / Maofu Fu ... [et al.] -- Two-dimensional gel electrophoresis for the identification of signaling targets / Yukihito Kabuyama ... [et al.] -- A high-throughput mammalian cell-based transient transfection assay / Daniel J. Noonan, Kenneth Henry, and Michelle L. Twaroski -- Determining protein half-lives / Pengbo Zhou -- Assaying protein kinase activity / Jan Brábek and Steven K. Hanks. Comparative phosphorylation site mapping from gel-derived proteins using a multidimensional ES/MS-based approach / Francesca Zappacosta, Michael J. Huddleston and Roland S. Annan -- Studies of calmodulin-dependent regulation / Paul C. Brandt and Thomas C. Vanaman -- Measurement of protein-DNA interactions in vivo by chromatin immunoprecipitation -- Hogune Im ... [et al.]. Characterization of protein-DNA association in vivo by chromatin immunoprecipitation / Laurent Kuras -- Nonradioactive methods for detecting activation of Ras-related small G proteins / Douglas A. Andres -- Nucleocytoplasmic glycosylation, O-GlcNAc: identification and site mapping / Natasha Elizabeth Zachara, Win Den Cheung, and Gerald Warren Hart. Techniques in protein methylation / Jaeho Lee, Donghang Cheng, and Mark T. Bedford -- Assaying lipid phosphate phosphatase activities / Gil-Soo Han and George M. Carman -- Assaying phosphoinositide phosphatases / Gregory S. Taylor and Jack E. Dixon -- Assaying phospholipase A² activity / Christina C. Leslie and Michael H. Gelb. Measurement and immunofluorescence of cellular phosphinositides / Hiroko Hama ... [et al.] -- Measuring dynamic changes in cAMP using fluorescence resonance energy transfer / Sandrine Evellin ... [et al.] -- In vivo detection of protein-protein interaction in plant cells using BRET / Chitra Subramanian ... [et al.] -- Revealing protein dynamics by photobleaching techniques / Frank van Drogen and Matthias Peter -- Assaying cytochrome c translocation during apoptosis / Nigel J. Waterhouse ... [et al.].