Epidermal cells : methods and protocols

Primary mouse keratinocyte culture / Annalisa Pirrone, Barbara Hager, and Philip Fleckman -- Serial cultivation of primary adult murine keratinocytes / Richard P. Redvers and Pritinder Kaur -- Keratinocyte culture in the absence of substrate attachment / Monika Jost and Ulrich Rodeck -- Application of genetically modified feeder cells for culture of keratinocytes / Takashi Kameda and Toshihiro Sugiyama -- Organ culture of developing mouse skin and its application for molecular mechanistic studies of morphogenesis / Mariko Kashiwaga and Nam-ho Huh -- Experimental models to analyze differentiation functions of cultured keratinocytes in vitro and in vivo / Nicole Maas-Szabowski, Norbert E. Fusenig, and Hans-Jürgen Stark -- In vitro fabrication of engineered human skin / Alexander Margulis, Weitian Zhang and Jonathan A. Garlick -- In vivo labeling and analysis of epidermal stem cells / Wei-yang Wu and Rebecca J. Morris -- Method for the harvest and assay of in vitro clonogenic keratinocytes stem cells from mice / Wei-yang Wu and Rebecca J. Morris -- FACS enrichment of human keratinocyte stem cells / Amy Li and Pritinder Kaur -- Isolation, characterization, and culture of epithelial stem cells / Jackie R. Bickenbach -- Keratin 19 as a stem cell marker in vivo and in vitro / Danielle Larouche [et al.] -- Immunolocalization in the epidermis / Tammy-Claire Troy [et al.] -- Epidermal cell analysis by RT-PCR / Tammy-Claire Troy [et al.] -- Whole-mount assays for gene induction and barrier formation in the developing epidermis / Carolyn Byrne and Matthew J. Hardman -- Analysis of early epidermal development in zebrafish / Ashley E. Webb and David Kimelman -- Analysis of E2F factors during epidermal differentiation / Wing Y. Chang and Lina Dagnino -- Analysis of HOX homeodomain proteins and gene transcripts -- Apoptosis in the epidermis / Kiyofumi Yamanishi, Chun-Shen Shen and Hitoshi Mizutani -- Fate of desmosomal proteins in apoptotic epidermal cells / J. Weiske and Otmar Huber -- Analysis of connexin 43 expression on keratinocytes using flow cytometry / Maja Matic [et al.] -- MMP-9 and TIMP-1 assays in keratinocyte cultures / Takashi Kobayashi -- Characterization of epithelial cells in the hair follicle with S100 proteins / Kenji Kizawa and Mayumi Ito -- Immunoelectron microscopic analysis of cornified cell envelopes and antigen retrieval / Akemi Ishida-Yamamoto -- Cell kinetic analysis in artificial skin using immunochemical methods / Andrea Casasco [et al.] -- Proliferation, differentiation, and inflammation in normal and hyperproliferative skin using multiparameter flow cytometry / Piet E. J. van Erp -- Fluorimetric DNA assay of cell number / William R. Otto -- Keratinocyte transient transfections / Anthony M. Flores and Brian J. Aneskievich -- Tetracycline-regulated gene expression in epidermal keratinocytes / Richard B. Presland and Philip Fleckman -- Gene targeting by oligonucleotides in keratinocytes / Olga Igoucheva and Kyonggeun Yoon -- Promoter analysis in the human SPRR gene family / David F. Fischer and Claude Backendorf -- Stable integration of large PAC contructs in keratinocytes / Sarah H. Williams and Alain Hovnanian -- Targeted somatic mutagenesis in the mouse epidermis / Daniel Metzger, Mei Li, and Pierre Chambon -- Methods to study protein/protein interactions / Jin-jun Meng [et al.] -- Isolation of recombinant phage displayed antibodies recognizing skin keratinocytes / Kim Bak Jensen and Peter Kristensen -- Analysis of tissue-specific DNA methylation during development / Jun Ohgane, Naka Hattori and Kunio Shiota -- Serial analysis of gene expression in human keratinocytes and the epidermis / Bastiaan J. H. Jansen [et al.] -- Methods for gene expression profiling in dermatology research using dermarray nylon filter DNA microarrays / Richard L. Davis, Jr. [et al.] -- Two-photon fluorescence imaging and reactive oxygen species detection within the epidermis / Kerry M. Hanson and Robert M. Clegg -- In vivo transplantation of engineered human skin / Shari Greenberg, Alexander Margulis and Jonathan A. Garlick -- Epidermis targeted gene transfer using in vivo electroporation / Hiroki Maruyama, Jun-ichi Miyazaki, and Fumitake Gejyo -- Gene and stem cell therapy of the hair follicle / Robert M. Hoffman