DNA viruses : methods and protocols

Viral detection -- Quantitative detection of Epstein-Barr virus DNA in clinical specimens by rapid real-time PCR targeting a highly conserved region of EBNA-1 -- Profiling of Epstein-Barr virus latent RNA expression in clinical specimens by gene-specific multiprimed cDNA synthesis and PCR -- Quantitative detection of viral gene expression in populations of Epstein-Barr virus-infected cells in vivo -- Detection and quantification of the rare latently infected cell undergoing herpes simplex virus transcriptional activation in the nervous system in vivo -- Reporter cell lines for the detection of herpes simplex viruses -- Unraveling the architecture of viruses by high-resolution atomic force microscopy -- Studying the structure of large viruses with multiresolution imaging -- Herpes simplex virus-cell interactions studied by low-fading contrasted immunofluorescence -- Herpes simplex virus-cell interactions studied by immunogold cryosection electron microscopy -- FTIR microscopy detection of cells infected with viruses -- The JC virus-like particle overlay assay -- Analysis of fusion using a virus-free cell fusion assay -- Pseudovirions as specific tools for investigation of virus interactions with cells -- Simultaneous in situ detection of RNA, DNA, and protein using tyramide-coupled immunofluorescence -- Identification and characterization of herpesviral immediate-early genes -- Methods for measuring the replication and segregation of Epstein-Barr virus-based plasmids -- DNA affinity purification of Epstein-Barr virus OriP-binding proteins. Pre-B-cell colony formation assay -- Luciferase real-time bioluminescence imaging for the study of viral pathogenesis -- Culturing primary and transformed neuronal cells for studying pseudorabies virus infection -- Human papillomavirus type 31 life cycle: methods for study using tissue culture models -- Molecular genetics of herpesviruses: a recombinant technology approach -- Molecular genetics of DNA viruses: recombinant virus technology -- Genetic analysis of cytomegalovirus by shuttle mutagenesis -- Construction of a gene inactivation library for Bovine herpesvirus 1 using infectious clone technology -- Selective silencing of viral gene E6 and E7 expression in HPV-positive human cervical carcinoma cells using small interfering RNAs -- Design of a herpes simplex virus type 2 long oligonucleotide-based microarray: global analysis of HSV-2 transcript abundance during productive infection -- Real-time quantitative PCR analysis of viral transcription -- Rapid screening of chemical inhibitors that block processive DNA synthesis of herpesviruses: potential application to high-throughput screening.