Human retrovirus protocols : virology and molecular biology

Enhanced culture assay for detection and quantitation of latently infected, resting cd4+ t-cells carrying replication-competent virus in hiv-1-infected individuals / J.D. Siliciano and R.F. Siliciano - - Isolation, propagation, and titration of human immunodeficiency virus type 1 from peripheral blood of infected individuals / H. Schuitemaker and N.A. Kootstra - - Isolation of human immunodeficiency virus type 1 from peripheral blood monocytes / P.R. Gorry ... [et al.] - - Isolation, propagation, and hiv-1 infection of monocyte-derived macrophages and recovery of virus from brain and cerebrospinal fluid / S. Gorantla, M. Che and H.E. Gendelman - - Isolation and hiv-1 infection of primary human microglia from fetal and adult tissue / K. Borgmann, H.E. Gendelman and A. Ghorpade - - Isolation of human immunodeficiency virus type 1 from semen and vaginal fluids / A.A. Kiessling - - Isolation and quantification of hiv from lymph nodes / C. Tamalet - - Isolation of human immunodeficiency virus type 2 biological clones from peripheral blood lymphocytes / H. Blaak - - Isolation and confirmation of human t-cell leukemia virus type 2 from peripheral blood mononuclear cells / M.D. Lairmore and A. Montgomery - - Isolation of foamy viruses from peripheral blood lymphocytes / J. Tobaly-Tapiero, P. Bittoun and A. Saib - - Alu-ltr real-time nested pcr assay for quantifying integrated hiv-1 DNA / A. Brussel, O. Delelis and P. Sonigo - - Quantification of hfv-integrated DNA in human cells by alu-ltr real-time pcr / O. Delelis, A. Brussel and P. Sonigo - - Detection of hiv-1 provirus and rna by in situ amplification / A. Nicol and G.J. Nuovo - - Detection of htlv-1 gene on cytologic smear slides / K. Kashima, T. Daa and S. Yokoyama - - Detection of hiv-2 by pcr / F. Gao - - Quantitation of hiv-1 viral rna in blood plasma and genital secretions / S.A. Fiscus - - Quantification of proviral DNA load of human immunodeficiency virus type 2 subtypes a and b using real-time pcr / M. Gueudin, F. Damond and F. Simon - - Plasma rna viral load in hiv-1 group o infection by real-time pcr / M. Gueudin and F. Simon - - A new combined hiv p24 antigen and anti-hiv-1/2/o screening assay / S. Polywka ... [et al.] - - A new automated fourth-generation hiv screening assay with sensitive antigen detection module and high specificity / B. Weber - - Detection of antiviral antibodies using enzyme-linked immunosorbent assay / I.G. Winkler - - Plaque-reduction assays for human and simian immunodeficiency virus neutralization / A. Nordqvist and E.M. Fenyo - - Detection of drug-resistant hiv-1 strains / A.A. Kiessling, S.J. Eyre and B.D. Desmarais - - Determination of cell tropism of hiv-1 / N.A. Kootstra and H. Schuitemaker - - Determination of co-receptor usage of hiv-1 / H. Schuitemaker and N.A. Kootstra - - Quantitative evaluation of hiv and siv co-receptor use with ghost(3) cell assay / D. Vodros and E.M. Fenyo - - Phenotypic characterization of blood monocytes from hiv-infected individuals / P.J. Ellery and S.M. Crowe - - Methods to determine hiv-1 ex vivo fitness / A. Abraha ... [et al.] - - A yeast recombination-based cloning system to produce chimeric hiv-1 viruses and express hiv-1 genes / D.M. Moore ... [et al.] - - Pcr amplification, cloning, and construction of hiv-1 infectious molecular clones from virtually full-length hiv-1 genomes / P.K. Ehrenberg and N.L. Michael - - Amplification and cloning of near full-length hiv-2 genomes / F. Gao - - Growth and manipulation of a human t-cell leukemia virus type 2 full-length molecular clone / M. Anderson and P.L. Green - - Construction and analysis of genomic, full-length infectious foamy virus DNA clones / R. Wirtz and M. Lochelt - - Molecular characterization of proteolytic processing of the gag proteins of human spumaretrovirus / K.I. Pfrepper and R.M. Flugel - - Assessing the relative efficacy of antiretroviral activity of different drugs on macrophages / S. Aquaro and C.F. Perno - - Gene expression profiling of hiv-1 infection using cdna microarrays / A.B. van 't Wout - - Determination of dc-sign and dc-signr repeat region variations / H. Liu and T. Zhu