2D PAGE : sample preparation and fractionation

v.1 : Pt I. Sample preparation basics : 1. Mechanical/physical methods of cell disruption and tissue homogenization / Stanley Goldberg -- 2. Bacteria and yeast cells disruption using lytic enzymes / Oriana Salazar -- 3. Sample solubilization buffers for two-dimensional electrophoresis / Walter Weiss -- 4. Quantitation of protein in samples prepared for 2-D electrophoresis / Thomas Berkelman -- 5. Removal of Interfering Substances in Samples Prepared for 2-D Electrophoresis / Thomas Berkelman -- 6. Protein Concentration by Hydrophilic Interaction Chromatography combined with Solid Phase Extraction / Ulrich Schneider -- Pt II. Protein labeling techniques : 7. Difference Gel Electrophoresis Based on Lys/Cys Tagging / Reiner Westermeier -- 8. Isotope-coded two-dimensional maps : tagging with deuterated acrylamide and 2- vinylpyridine / Pier Giorgio Righetti -- 9. Stable Isotope Labeling by Amino acids in Cell culture (SILAC) / Albrecht Gruhler -- 10. ICPL - Isotope-coded protein label / Josef Kellermann -- 11. Radiolabeling for Two-Dimensional Gel Analysis / Hélian Boucherie -- Pt. III. Fractionation of proteins by chemical reagents and chromatography : 12. Sequential extraction of proteins by chemical reagents / Stuart Cordwell -- 13. Reducing sample complexity by RP-HPLC : beyond the tip of the protein expression iceberg / Gert van den Bergh -- 14. Enriching Basic and Acidic Rat Brain Proteins with Ion Exchange Mini Spin Columns Prior To Two-Dimensional Gel Electrophoresis / Ning Liu -- 15. Reducing the complexity of the Escherichia coli proteome by chromatography on reactive dye columns / Phil Cash -- 16. Reducing sample complexity in Proteomics by Chromatofocusing with simple buffer mixtures / Douglas D. Frey -- 17. Fractionation of Proteins by Immobilized Metal Affinity Chromatography / Qing-Yu He -- 18. Fractionation of proteins by heparin chromatography / Qing-Yu He -- Pt. IV. Fractionation of proteins by electrophoresis methods : 19. Fractionation of Complex Protein Mixtures By Liquid-Phase Isoelectric Focusing / Julie Hey -- 20. Microscale isoelectric focusing in solution : a method for comprehensive and quantitative proteome analysis Using 1-D and 2-D DIGE combined with MicroSol IEF prefractionation / David Speicher -- 21. Prefractionation, enrichment, desalting and depleting of low volume and low abundance proteins and peptides using the MF10 / Valerie Wasinger -- 22. Sample prefractionation in Granulated Sephadex IEF Gels / Angelika Görg -- 23. Free-Flow Electrophoresis System for Plasma Proteomic Applications / Robert Wildgruber -- 24. Protein fractionation by preparative electrophoresis / Michael Fountoulakis -- Pt. V. Enrichment strategies for organelles, multiprotein complexes and specific protein classes : 25. Isolation of endocitic organelles by density gradient centrifugation / Lukas A. Huber -- 26. Isolation of highly pure rat liver mitochondria with the aid of zone-electrophoresis in a free flow device (ZE-FFE) / Hans Zischka -- 27. Isolation of Proteins and Protein Complexes by Immunoprecipitation / Barbara Kaboord -- 28. Isolation of Phosphoproteins / Lynn A. Megeney -- 29. Glycoprotein Enrichment through Lectin Affinity Techniques / Milos V. Novotny -- 30. Isolation of Bacterial Cell Membranes Proteins using Carbonate Extraction / Mark Molloy -- 31. Enrichment of Membrane Proteins by Partitioning in Detergent/Polymer Aqueous Two-Phase Systems / Folke Tjerneld -- 32. The isolation of detergent-resistant lipid rafts for two-dimensional electrophoresis / Young-Gyu Ko -- 33. Isolation of membrane protein complexes by Blue Native Electrophoresis / Lutz A. Eichacker -- 34. Tissue Microdissection / Michael R. Buck. v. 2. Application of fluorescence dye saturation labeling for differential proteome analysis of 1,000 microdissected cells from pancreatic ductal adenocarcinoma precursoe lesions / Barbara Sitek ... [et al.] -- Albumin and immunoglobulin depletion of human plasma / Rosalind e. Jenkins ... [et al.] -- Multi-component immunoaffinity subtraction and reversed-phase chromatography of human serum / James Martosella and Nina Zolotarjova -- Immunoaffinity fractionation of plasma proteins by chicken IgY antibodies / Lei Huang and Xiangming Fang -- Proteomics of cerebrospinal fluid methods for sample processing / John E. Hale ... [et al.] -- Sample preparation of bronchoalveolar lavage fluid / Baptiste Leroy, Paul Falmagne, and Ruddy Wattiez -- Preparation of nasal secretions for peoteome analysis / Begona Casado, Paolo Iadarola, and Lewis K. Pannell -- Preparation of urine samples for proteomic analysis / Rembert Pieper -- Isolation of cytoplasmatic proteins from cultures cells for two-dimensional gel electrophoresis / Ying Wang, Jen-Fu Chiu, and Qing-Yu He -- Sample preparation of culture medium from Madin-Darby Canine Kidney cells / Daniel Ambort, Daniel Lottaz, and Erwin Sterchi -- Sample preparation for mass spectromentry analysis of formalin-fixed paraffin-embedded tissue proteomic analysis of formalin-fixed tissue / Nicolas A. Stewart and Timothy D. Veenstra -- Metalloproteomics in the molecular study of cell physiology and disease / Hermann-Josef Thierse, Stefanie Helm, and Patrick Pankert -- Protein extraction from green plant tissue / Ragnar Flengsrud -- The terminator a device for high-throughput extraction of plant material / B. M. van den Berg -- Isolation of mitochondria from plant cell culture / Etienne H. Meyer and A. Harvey Millar -- Isolation and preparation of choroplasts from Arabidopsis thaliana plants / Sybille E. Kubis, Kathryn S. Lilley, and Paul Jarvis -- Isolation of plant cell wall proteins / Elisabeth Jamet ... [et al.] -- Isolation and fractionation of the endoplasmic reticulum from castor bean (Ricinius communis) endosperm for proteomic analyses / William J. Simon, Daniel J. Maltman, and Antoni R. Slabas -- Cell wall fractionation for yeast and fungal proteomics / Aida Pitarch, Césa Nombela, and Concha Gil -- Sample preparation procedure for cellular fungi / Alois Harder -- Isolation and enrichment of secreted proteins from filamentous fungi / Martha L. Medina and Wilson A. Frnacisco -- Isolation and solubilization of cellular membrane proteins from bacteria / Kheir Zuobi-Hasona and L. Jeannine Brady -- Isolation and solubilization of gram-positive bacterial cell wall-associated proteins / Jason N. Cole, Steven P. Djordjevic, and Mark J. Walker -- Cell fractionation of parasitic protozoa / Wanderley de Souza, José Andrés-Morgado-Diaz, and Narcisa L. Cunha-e-Silva