Preview this item Preview this item More like this Subjects Biomolecules -- Separation. Biotechnology -- Technique. Proteins -- isolation and purification. View all subjects Similar ItemsDownstream processing of proteins : methods and protoc

1 Downstream Processing in the Biotechnology Industry: An Overview Manohar Kalyanpur ............................................................................... 1 2 Microbial Cell Disruption by High-Pressure Homogenization Anton P. J. Middelberg........................................................................ 11 3 Protein Concentration and Buffer Exchange Using Ultrafiltration Andrew L. Zydney and Ralf Kuriyel................................................... 23 4 High-Performance Tangential Flow Filtration for Protein Separations Andrew L. Zydney and Ralf Kuriyel................................................... 35 5 Large-Scale Recovery of Protein Inclusion Bodies by Continuous Centrifugation Anton P. J. Middelberg........................................................................ 47 6 Application of Density Gradient Ultracentrifugation Using Zonal Rotors in the Large-Scale Purification of Biomolecules Mohamed A. Desai and Sandra P. Merino ........................................ 59 7 Application of Chromatography in the Downstream Processing of Biomolecules Mohamed A. Desai, Mark Rayner, Mike Burns, and Dervilla Bermingham ...................................................................... 73 8 Affinity Ligand Technology: Optimal Protein Separations for Downstream Processing Ken Jones and Dev Baines ................................................................ 95 9 Immunoaffinity Adsorption in the Large-Scale Isolation of Biomolecules Mohamed A. Desai ............................................................................. 109 10 Expanded Bed Adsorption in the Purification of Biomolecules Allan Lihme, Marie Hansen, Morten Olander, and Elias Zafirakos....................................................................... 121 11 Application of HPLC in the Purification of Biomolecules Paul Bradley and Mohamed A. Desai .............................................. 141 vii viii Contents 12 Countercurrent Chromatography of Proteins with Polymer Phase Systems Yoichiro Shibusawa, Kazuhiro Matsuda, and Yoichiro Ito........... 161 13 Practical Aspects and Applications of Radial Flow Chromatography Denise M. Wallworth .......................................................................... 173 14 Sterile Filtration and Virus Filtration Ralf Kuriyel and Andrew L. Zydney ................................................. 185 15 Design and Interpretation of Viral Clearance Studies for Biopharmaceutical Products Allan J. Darling ................................................................................... 195 16 Validation of Sterilizing Filters in the Biotechnology Industry Manohar Kalyanpur ........................................................................... 211 Index ......................................................................................................... 223